Differentiation of human promyelocytic leukemia cells in vitro by 6-thioguanine

Rose J. Papac; Alfred E. Brown; Edward L. Schwartz; Alan C. Sartorelli

doi:10.1016/0304-3835(80)90062-2

Differentiation of human promyelocytic leukemia cells in vitro by 6-thioguanine

Rose J. Papac, Alfred E. Brown, Edward L. Schwartz, Alan C. Sartorelli

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The antineoplastic agent, 6-thiogaunine, was found to be a highly potent inducer of differentiation of HL60 human leukemia cells in vitro, producing cells with predominantly myeloid characteristics, as judged by morphological, functional, and cytochemical criteria. Maturation of HL60 was attainable without significant cytotoxicity as measured by cell number; moreover, maximal differentiation occurred at a level of the purine antimetabolite below that producing 50% growth inhibition. The 6-thioguanine-induced differentiated cells had distinctive granulocytic morphology, and were capable of generating superoxide anion during the respiratory burst typical of mature phagocytic cells.

Original language	English (US)
Pages (from-to)	33-38
Number of pages	6
Journal	Cancer Letters
Volume	10
Issue number	1
DOIs	https://doi.org/10.1016/0304-3835(80)90062-2
State	Published - Jul 1980
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0304-3835(80)90062-2

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title = "Differentiation of human promyelocytic leukemia cells in vitro by 6-thioguanine",

abstract = "The antineoplastic agent, 6-thiogaunine, was found to be a highly potent inducer of differentiation of HL60 human leukemia cells in vitro, producing cells with predominantly myeloid characteristics, as judged by morphological, functional, and cytochemical criteria. Maturation of HL60 was attainable without significant cytotoxicity as measured by cell number; moreover, maximal differentiation occurred at a level of the purine antimetabolite below that producing 50% growth inhibition. The 6-thioguanine-induced differentiated cells had distinctive granulocytic morphology, and were capable of generating superoxide anion during the respiratory burst typical of mature phagocytic cells.",

author = "Papac, {Rose J.} and Brown, {Alfred E.} and Schwartz, {Edward L.} and Sartorelli, {Alan C.}",

note = "Funding Information: *Supported in part by U.S. Public Health Service Grants CA-62817 and CA-16369 from the National Cancer Institute and a grant from the Bristol-Myers Company. E.L.S. is a fellow in Cander Research supported by Grant DRG-408F of the Damon Runyon-Waiter Winchell Cancer Fund.",

year = "1980",

month = jul,

doi = "10.1016/0304-3835(80)90062-2",

language = "English (US)",

volume = "10",

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journal = "Cancer Letters",

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TY - JOUR

T1 - Differentiation of human promyelocytic leukemia cells in vitro by 6-thioguanine

AU - Papac, Rose J.

AU - Brown, Alfred E.

AU - Schwartz, Edward L.

AU - Sartorelli, Alan C.

N1 - Funding Information: *Supported in part by U.S. Public Health Service Grants CA-62817 and CA-16369 from the National Cancer Institute and a grant from the Bristol-Myers Company. E.L.S. is a fellow in Cander Research supported by Grant DRG-408F of the Damon Runyon-Waiter Winchell Cancer Fund.

PY - 1980/7

Y1 - 1980/7

N2 - The antineoplastic agent, 6-thiogaunine, was found to be a highly potent inducer of differentiation of HL60 human leukemia cells in vitro, producing cells with predominantly myeloid characteristics, as judged by morphological, functional, and cytochemical criteria. Maturation of HL60 was attainable without significant cytotoxicity as measured by cell number; moreover, maximal differentiation occurred at a level of the purine antimetabolite below that producing 50% growth inhibition. The 6-thioguanine-induced differentiated cells had distinctive granulocytic morphology, and were capable of generating superoxide anion during the respiratory burst typical of mature phagocytic cells.

AB - The antineoplastic agent, 6-thiogaunine, was found to be a highly potent inducer of differentiation of HL60 human leukemia cells in vitro, producing cells with predominantly myeloid characteristics, as judged by morphological, functional, and cytochemical criteria. Maturation of HL60 was attainable without significant cytotoxicity as measured by cell number; moreover, maximal differentiation occurred at a level of the purine antimetabolite below that producing 50% growth inhibition. The 6-thioguanine-induced differentiated cells had distinctive granulocytic morphology, and were capable of generating superoxide anion during the respiratory burst typical of mature phagocytic cells.

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Differentiation of human promyelocytic leukemia cells in vitro by 6-thioguanine

Abstract

ASJC Scopus subject areas

UN SDGs

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