TY - JOUR
T1 - X-ray radiosensitivity of brain glioma stem cells and U251 cells
AU - Fan, Ruitai
AU - Liu, Junqi
AU - Zhang, Hongzhi
AU - Ge, Chenlei
AU - Hu, Yong
AU - Guan, Fangxia
AU - Yang, Bo
PY - 2011/4/15
Y1 - 2011/4/15
N2 - Objective: To study the radiosensitivity of brain glioma stem cells and U251 glioma cells to X-rays at different doses. Methods: Glioma tumor spheres were separated and cultured from glioma tissues, and glioma stem cells were obtained through CD133 immunomagnetic bead separation. After radiation was administered to the glioma stem cells and U251 glioma cells at different doses, the radiosensitivity of these cells was detected by colony formation assays. Results: As the X-ray dose increased, the survival rate of the two cell lines decreased. However, the survival rate of glioma stem cells was significantly higher than that of U251 cells ( t = -3.71, P < 0.01). The experimental data were fitted according to the formula: SF = exp (-αD-βD2), and α / β values of the tumor stem cells and U251 cells were 3.57 and 7.15, respectively. Conclusion: Glioma stem cells have a stronger resistance to radiation than U251 glioma cells.
AB - Objective: To study the radiosensitivity of brain glioma stem cells and U251 glioma cells to X-rays at different doses. Methods: Glioma tumor spheres were separated and cultured from glioma tissues, and glioma stem cells were obtained through CD133 immunomagnetic bead separation. After radiation was administered to the glioma stem cells and U251 glioma cells at different doses, the radiosensitivity of these cells was detected by colony formation assays. Results: As the X-ray dose increased, the survival rate of the two cell lines decreased. However, the survival rate of glioma stem cells was significantly higher than that of U251 cells ( t = -3.71, P < 0.01). The experimental data were fitted according to the formula: SF = exp (-αD-βD2), and α / β values of the tumor stem cells and U251 cells were 3.57 and 7.15, respectively. Conclusion: Glioma stem cells have a stronger resistance to radiation than U251 glioma cells.
KW - CD133 immunomagnetic beads
KW - Glioma stem cell
KW - Radiosensitivity
KW - U251 glioma cell line
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U2 - 10.3969/j.issn.1000-8179.2011.07.007
DO - 10.3969/j.issn.1000-8179.2011.07.007
M3 - Article
AN - SCOPUS:79955932719
SN - 1000-8179
VL - 38
SP - 391
EP - 393
JO - Chinese Journal of Clinical Oncology
JF - Chinese Journal of Clinical Oncology
IS - 7
ER -
