Wilms’ tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation

David M. Loeb; Ella Evron; Chirag B. Patel; Prem Mohini Sharma; Birunthi Niranjan; Lakjaya Buluwela; Sigmund A. Weitzman; Dorian Korz; Saraswati Sukumar

Wilms’ tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation

David M. Loeb, Ella Evron, Chirag B. Patel, Prem Mohini Sharma, Birunthi Niranjan, Lakjaya Buluwela, Sigmund A. Weitzman, Dorian Korz, Saraswati Sukumar

Research output: Contribution to journal › Article › peer-review

205 Scopus citations

Abstract

We analyzed Wilms’ tumor suppressor 1 (WT1) expression and its regulation by promoter methylation in a panel of normal breast epithelial samples and primary carcinomas. Contrary to previous reports, WT1 protein was strongly expressed in primary carcinomas (27 of 31 tumors) but not in normal breast epithelium (1 of 20 samples). Additionally, the WT1 promoter was methylated in 6 of 19 (32%) primary tumors, which nevertheless expressed WT1. The promoter is not methylated in normal epithelium. Thus, although tumor-specific methylation of WT1 is established in primary breast cancer at a low frequency, other transcriptional regulatory mechanisms appear to supercede its effects in these tumors. Our results demonstrate expression of WT1 in mammary neoplasia, and that WT1 may not have a tumor suppressor role in breast cancer.

Original language	English (US)
Pages (from-to)	921-925
Number of pages	5
Journal	Cancer research
Volume	61
Issue number	3
State	Published - Feb 1 2001
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

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Cite this

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title = "Wilms{\textquoteright} tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation",

abstract = "We analyzed Wilms{\textquoteright} tumor suppressor 1 (WT1) expression and its regulation by promoter methylation in a panel of normal breast epithelial samples and primary carcinomas. Contrary to previous reports, WT1 protein was strongly expressed in primary carcinomas (27 of 31 tumors) but not in normal breast epithelium (1 of 20 samples). Additionally, the WT1 promoter was methylated in 6 of 19 (32%) primary tumors, which nevertheless expressed WT1. The promoter is not methylated in normal epithelium. Thus, although tumor-specific methylation of WT1 is established in primary breast cancer at a low frequency, other transcriptional regulatory mechanisms appear to supercede its effects in these tumors. Our results demonstrate expression of WT1 in mammary neoplasia, and that WT1 may not have a tumor suppressor role in breast cancer.",

author = "Loeb, {David M.} and Ella Evron and Patel, {Chirag B.} and Sharma, {Prem Mohini} and Birunthi Niranjan and Lakjaya Buluwela and Weitzman, {Sigmund A.} and Dorian Korz and Saraswati Sukumar",

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T1 - Wilms’ tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation

AU - Loeb, David M.

AU - Evron, Ella

AU - Patel, Chirag B.

AU - Sharma, Prem Mohini

AU - Niranjan, Birunthi

AU - Buluwela, Lakjaya

AU - Weitzman, Sigmund A.

AU - Korz, Dorian

AU - Sukumar, Saraswati

PY - 2001/2/1

Y1 - 2001/2/1

N2 - We analyzed Wilms’ tumor suppressor 1 (WT1) expression and its regulation by promoter methylation in a panel of normal breast epithelial samples and primary carcinomas. Contrary to previous reports, WT1 protein was strongly expressed in primary carcinomas (27 of 31 tumors) but not in normal breast epithelium (1 of 20 samples). Additionally, the WT1 promoter was methylated in 6 of 19 (32%) primary tumors, which nevertheless expressed WT1. The promoter is not methylated in normal epithelium. Thus, although tumor-specific methylation of WT1 is established in primary breast cancer at a low frequency, other transcriptional regulatory mechanisms appear to supercede its effects in these tumors. Our results demonstrate expression of WT1 in mammary neoplasia, and that WT1 may not have a tumor suppressor role in breast cancer.

AB - We analyzed Wilms’ tumor suppressor 1 (WT1) expression and its regulation by promoter methylation in a panel of normal breast epithelial samples and primary carcinomas. Contrary to previous reports, WT1 protein was strongly expressed in primary carcinomas (27 of 31 tumors) but not in normal breast epithelium (1 of 20 samples). Additionally, the WT1 promoter was methylated in 6 of 19 (32%) primary tumors, which nevertheless expressed WT1. The promoter is not methylated in normal epithelium. Thus, although tumor-specific methylation of WT1 is established in primary breast cancer at a low frequency, other transcriptional regulatory mechanisms appear to supercede its effects in these tumors. Our results demonstrate expression of WT1 in mammary neoplasia, and that WT1 may not have a tumor suppressor role in breast cancer.

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Wilms’ tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation

Abstract

ASJC Scopus subject areas

UN SDGs

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