VSV-Displayed HIV-1 Envelope Identifies Broadly Neutralizing Antibodies Class-Switched to IgG and IgA

Manxue Jia, Rachel A. Liberatore, Yicheng Guo, Kun Wei Chan, Ruimin Pan, Hong Lu, Eric Waltari, Eva Mittler, Kartik Chandran, Andrés Finzi, Daniel E. Kaufmann, Michael S. Seaman, David D. Ho, Lawrence Shapiro, Zizhang Sheng, Xiang Peng Kong, Paul D. Bieniasz, Xueling Wu

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


The HIV-1 envelope (Env) undergoes conformational changes during infection. Broadly neutralizing antibodies (bNAbs) are typically isolated by using soluble Env trimers, which do not capture all Env states. To address these limitations, we devised a vesicular stomatitis virus (VSV)-based probe to display membrane-embedded Env trimers and isolated five bNAbs from two chronically infected donors, M4008 and M1214. Donor B cell receptor (BCR) repertoires identified two bNAb lineages, M4008_N1 and M1214_N1, that class-switched to immunoglobulin G (IgG) and IgA. Variants of these bNAbs reconstituted as IgA demonstrated broadly neutralizing activity, and the IgA fraction of M1214 plasma conferred neutralization. M4008_N1 epitope mapping revealed a glycan-independent V3 epitope conferring tier 2 virus neutralization. A 4.86-Å-resolution cryogenic electron microscopy (cryo-EM) structure of M1214_N1 complexed with CH505 SOSIP revealed another elongated epitope, the V2V5 corridor, extending from V2 to V5. Overall, the VSVENV probe identified bNAb lineages with neutralizing IgG and IgA members targeting distinct sites of HIV-1 Env vulnerability.

Original languageEnglish (US)
Pages (from-to)963-975.e5
JournalCell Host and Microbe
Issue number6
StatePublished - Jun 10 2020


  • Env immunogen design
  • HIV vaccine
  • IgG to IgA
  • V2V5 corridor
  • antibody class-switch
  • broadly neutralizing antibody
  • cryo-EM

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology


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