@inbook{7129ae3caaa04faf8125a1dcda6850ca,
title = "Viral delivery of GFP-dependent recombinases to the mouse brain",
abstract = "Many genetic tools have been developed that use green fluorescent protein (GFP) and its derivatives for labeling specific cell populations in organisms and in cell culture. To extend the use of GFP beyond labeling purposes, we developed methods and reagents that use GFP as a driver of biological activities. We used nanobodies that bind GFP to engineer CRE-DOG and Flp-DOG, recombinases that can induce Cre/lox and Flp/FRT recombination in a GFP-dependent manner, respectively. Here, we present a protocol to deliver CRE-DOG and Flp-DOG into the mouse brain by recombinant AAV infection. This protocol enables one to manipulate gene expression specifically in GFP-expressing cells, found either in transgenic GFP reporter lines or in cells made to express GFP by other transduction methods.",
keywords = "Adeno-associated viruses, Cell type-specific manipulation, Green fluorescent protein, Transgenic mouse",
author = "Tang, {Jonathan C.Y.} and Stephanie Rudolph and Cepko, {Constance L.}",
note = "Funding Information: We thank Wang Chen, Sophia Zhao, and Ru Xiao for help with viral preparation, Iain Drew for help with brain injections and optimizing the brain injection protocol, Emily Ellis for help with brain injections and tissue processing, the Harvard Medical School Neu-robiology Imaging Facility (supported by NINDS P30 Core Center grant NS072030) for consultation and instrument availability, Wade G. Regehr (W.G.R.) for research support, and Abigail Polter for comments on the manuscript. This work was funded by the Howard Hughes Medical Institute (C.L.C.) and the National Institutes of Health (F32 NS087708 to S.R., R01 NS32405 and R01 NS092707 to W.G.R). Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media LLC.",
year = "2017",
doi = "10.1007/978-1-4939-7169-5_8",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "109--126",
booktitle = "Methods in Molecular Biology",
}