TY - JOUR
T1 - Vaccination with a gE-negative bovine herpesvirus type 1 vaccine confers insufficient protection to a bovine herpesvirus type 5 challenge
AU - Silva, Alessandra D.
AU - Spilki, Fernando R.
AU - Franco, Ana Cláudia
AU - Esteves, Paulo A.
AU - Hübner, Silvia O.
AU - Driemeier, David
AU - Oliveira, Anna Paula
AU - Rijsewijk, Frans
AU - Roehe, Paulo M.
N1 - Funding Information:
We thank Dr L. Lovato, Dr C. Canal and Dr A. Simonetti for critically reviewing the manuscript. A.D. Silva and P.A. Esteves are students from the Programa de Pós-Graduação em Ciências Veterinárias da Faculdade de Veterinária (PPGCV/UFRGS/Brazil) grantees of CAPES. P.M. Roehe is a CNPq 1A research fellow. Work supported by CNPq, PRONEX, FAPERGS and CAPES.
PY - 2006/4/12
Y1 - 2006/4/12
N2 - In the present study, cross-protection to bovine herpesvirus type 5 (BHV-5) induced by bovine herpesvirus type 1 (BHV-1) vaccination was examined following inoculation of rabbits and calves with a glycoprotein E (gE)-negative BHV-1 vaccine and subsequent challenge with BHV-5. Rabbits (n = 5) and calves (n = 8) were vaccinated [five rabbits intranasally (IN), four calves IN and four intramuscularly (IM)] with 7.1 log10median tissue culture infective dose (TCID50) of the BHV-1 vaccine. Rabbits and calves were challenged IN [rabbits 2 weeks post-vaccination (pv); calves 5 weeks pv] with 9.1 log10 TCID50 of BHV-5. Two out of five vaccinated rabbits died after challenge with typical BHV-5 disease, as did 3/5 non-vaccinated controls. In calves, 4/8 vaccinated animals displayed mild signs of disease, whereas 6/6 non-vaccinated controls developed signs of disease, so severe that 2/6 had to be killed. Besides, nasal virus shedding post-challenge was not reduced by vaccination. At necropsy, on day 21 post-challenge, typical BHV-5 lesions were evident in brain tissues of both vaccinated and non-vaccinated calves. Dexametasone administration at 180 days post-infection did not reactivate clinical signs despite BHV-5 shedding in nasal secretions of both vaccinated and non-vaccinated calves. These results show that the BHV-1 vaccine evaluated here did not confer protection to BHV-5 in rabbits. In calves, BHV-1 vaccination did confer some protection to BHV-5 induced clinical disease, but it did not prevent infection and had no effect on nasal virus shedding or on the development of encephalitic lesions.
AB - In the present study, cross-protection to bovine herpesvirus type 5 (BHV-5) induced by bovine herpesvirus type 1 (BHV-1) vaccination was examined following inoculation of rabbits and calves with a glycoprotein E (gE)-negative BHV-1 vaccine and subsequent challenge with BHV-5. Rabbits (n = 5) and calves (n = 8) were vaccinated [five rabbits intranasally (IN), four calves IN and four intramuscularly (IM)] with 7.1 log10median tissue culture infective dose (TCID50) of the BHV-1 vaccine. Rabbits and calves were challenged IN [rabbits 2 weeks post-vaccination (pv); calves 5 weeks pv] with 9.1 log10 TCID50 of BHV-5. Two out of five vaccinated rabbits died after challenge with typical BHV-5 disease, as did 3/5 non-vaccinated controls. In calves, 4/8 vaccinated animals displayed mild signs of disease, whereas 6/6 non-vaccinated controls developed signs of disease, so severe that 2/6 had to be killed. Besides, nasal virus shedding post-challenge was not reduced by vaccination. At necropsy, on day 21 post-challenge, typical BHV-5 lesions were evident in brain tissues of both vaccinated and non-vaccinated calves. Dexametasone administration at 180 days post-infection did not reactivate clinical signs despite BHV-5 shedding in nasal secretions of both vaccinated and non-vaccinated calves. These results show that the BHV-1 vaccine evaluated here did not confer protection to BHV-5 in rabbits. In calves, BHV-1 vaccination did confer some protection to BHV-5 induced clinical disease, but it did not prevent infection and had no effect on nasal virus shedding or on the development of encephalitic lesions.
KW - Bovine herpesvirus type 1
KW - Bovine herpesvirus type 5
KW - Immunization
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U2 - 10.1016/j.vaccine.2006.01.024
DO - 10.1016/j.vaccine.2006.01.024
M3 - Article
C2 - 16480790
AN - SCOPUS:33645050664
SN - 0264-410X
VL - 24
SP - 3313
EP - 3320
JO - Vaccine
JF - Vaccine
IS - 16
ER -