TY - JOUR
T1 - Use of streptokinase as a recall antigen for lymphocyte proliferation testing in vitro
AU - Youkeles, Lisa H.
AU - Soliman, Mohammed Y.
AU - Rosenstreich, David L.
N1 - Funding Information:
From the Departments of Medicine and Microbiology and Immu-nology, Albert Einstein Collegee of Medicine, Bronx, N. Y. Supported by National Institutes of Health Grant AI-29871-01, National Institute of General Medical SciencesG rant T32 GM-7288, Albert Einstein College of Medicine Core Grant P30-CA-13330, and funds from the Saul and Lola Kramer Foundation, the G. Harold and Leila Y. Mathers Foundation,a nd the Irvington House Foundation. Received for publication Sept. 4, 1990. Revised Jan. 24, 1991. Accepted for publication Feb. 1, 1991. Reprint requests:D avid L. Rosenstreich,M D, Depts. of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461.
PY - 1991/8
Y1 - 1991/8
N2 - The use of streptokinase (SK) as a recall antigen (Ag) for stimulation of human lymphocyte proliferation in vitro was evaluated. SK is a potent stimulant, inducing a greater response than three other Ags, tetanus toxoid, Candida albicans extract, or streptolysin. The optimum stimulatory concentration is 1000 U/ml, and peak stimulation occurs between days 6 and 8. Human cord-blood lymphocytes do not respond to SK, suggesting that it is an Ag and not a mitogen. The response rate in an adult population was 82%, higher than the rate of two other Ags tested (tetanus toxoid or Candida). These findings demonstrate the ability of SK to act as a potent recall Ag for human lymphocyte proliferation in vitro.
AB - The use of streptokinase (SK) as a recall antigen (Ag) for stimulation of human lymphocyte proliferation in vitro was evaluated. SK is a potent stimulant, inducing a greater response than three other Ags, tetanus toxoid, Candida albicans extract, or streptolysin. The optimum stimulatory concentration is 1000 U/ml, and peak stimulation occurs between days 6 and 8. Human cord-blood lymphocytes do not respond to SK, suggesting that it is an Ag and not a mitogen. The response rate in an adult population was 82%, higher than the rate of two other Ags tested (tetanus toxoid or Candida). These findings demonstrate the ability of SK to act as a potent recall Ag for human lymphocyte proliferation in vitro.
KW - Streptokinase
KW - antigens
KW - cell-mediated immunity
KW - cord blood
KW - lymphocyte proliferation
KW - lymphocytes
KW - mitogens
KW - tetanus toxoid
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U2 - 10.1016/0091-6749(91)90324-H
DO - 10.1016/0091-6749(91)90324-H
M3 - Article
C2 - 1880316
AN - SCOPUS:0026055003
SN - 0091-6749
VL - 88
SP - 166
EP - 171
JO - The Journal of Allergy and Clinical Immunology
JF - The Journal of Allergy and Clinical Immunology
IS - 2
ER -