Two-dimensional DNA electrophoresis: state of the art and applications

Vincenzo Romano-Spica, Jan Vijg

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Abstract: Two-dimensional DNA electrophoresis allows the analysis of multiple DNA fragments based on both their size and sequence properties. The often complex gel separation patterns represent information-rich data sets that may be analyzed with dedicated image analysis systems. The introduction of a denaturing gradient in the second dimension enhances the capability of the method to identify sequence variations, and the recent construction of automated instruments opens up new ways for the diffusion of the procedure into the laboratory routine. In this review, the state of the art of the technique, its general principles and basic methodology will be discussed together with major applications in different fields, including environmental microbiology, mutation detection and human genetics.

Original languageEnglish (US)
Pages (from-to)1-30
Number of pages30
JournalBiotechnology Annual Review
Issue numberC
StatePublished - 1997
Externally publishedYes


  • DNA fingerprinting
  • DNA polymorphism
  • denaturing gradient gel electrophoresis (DGGE)
  • gene mutation
  • gene scanning
  • genetic epidemiology
  • genetic typing
  • genetic variation
  • genetics
  • genome scanning
  • genotoxicity
  • heteroduplex analysis
  • hospital infection
  • linkage
  • microbiology
  • mutation detection
  • mutational spectroscopy
  • polymerase chain reaction
  • restriction fragment length polymorphism
  • tumor analysis
  • two-dimensional DNA electrophoresis

ASJC Scopus subject areas

  • Biotechnology


Dive into the research topics of 'Two-dimensional DNA electrophoresis: state of the art and applications'. Together they form a unique fingerprint.

Cite this