Tumor antigen presentation by dermal antigen-presenting cells

Kristina Campton, Wanhong Ding, Zengmin Yan, Hiroaki Ozawa, Kristina Seiffert, Edward Miranda, Antonietta Lonati, Stefan Beissert, Richard D. Granstein

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Several phenotypes of antigen-presenting cells are present in the dermis, where they presumably function to present encountered antigens for immune responses. This study examined the ability of dermal antigen- presenting cells to present tumor-associated antigens for the induction of in vivo antitumor immunity. Total murine dermal cells were exposed either to medium alone or to medium containing tumor-associated antigens from S1509a tumor cells. Subsequently, dermal cells were injected subcutaneously at weekly intervals into naive mice for a total of three immunizations. One week following the final immunization, mice were challenged with living tumor cells. In these experiments, dermal cells pulsed with tumor-associated antigens induced protective immunity to tumor growth. Dermal cells exposed to tumor-associated antigens were also able to elicit delayed-type hypersensitivity after footpad injection into mice previously immunized against S1509a tumor cells. The ability to present tumor-associated antigens for both induction of antitumor immunity and elicitation of delayed-type hypersensitivity was dependent on I-A+ cells and was genetically restricted. Finally, dermal cells tended towards eliciting a greater antitumor delayed- type hypersensitivity response than epidermal cells. These results show that the murine dermis contains antigen-presenting cells capable of processing S1509a tumor antigens for the generation of protective antitumor immunity in vivo.

Original languageEnglish (US)
Pages (from-to)57-61
Number of pages5
JournalJournal of Investigative Dermatology
Issue number1
StatePublished - 2000
Externally publishedYes


  • Antigen presentation
  • Dendritic cells
  • Immuno-therapy
  • Tumor immunity

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology


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