Trypanosoma cruzi: Colony formation and clonal growth in agar

Murray Wittner; Linda Squillante; Jeffrey P. Nadler; Herbert B. Tanowitz

doi:10.1016/0014-4894(82)90067-4

Trypanosoma cruzi: Colony formation and clonal growth in agar

Murray Wittner, Linda Squillante, Jeffrey P. Nadler, Herbert B. Tanowitz

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Trypanosoma cruzi exhibited colonial growth when incorporated into 0.5-0.6% agar. Colonies were established from a single organism and clones readily derived. The plating efficiencies were variable depending on the original inoculum but were consistently over 50% when 10⁴ to 10⁵ parasites were added. The use of this technique for evaluation of an antitrypanosomal agent, nifurtimox, was demonstrated, making possible large-scale testing of potential antitrypanosomal agents and assessment of microbicidal and microbio-static drug levels.

Original language	English (US)
Pages (from-to)	255-261
Number of pages	7
Journal	Experimental Parasitology
Volume	53
Issue number	2
DOIs	https://doi.org/10.1016/0014-4894(82)90067-4
State	Published - Apr 1982
Externally published	Yes

Keywords

Agar medium
Chagas' disease
Cloning
Colony formation
Drug screening
Hemoflagellate
Nifurtimox
Protozoa
Trypanosoma cruzi
parasitic

ASJC Scopus subject areas

Parasitology
Immunology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0014-4894(82)90067-4

Cite this

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title = "Trypanosoma cruzi: Colony formation and clonal growth in agar",

abstract = "Trypanosoma cruzi exhibited colonial growth when incorporated into 0.5-0.6% agar. Colonies were established from a single organism and clones readily derived. The plating efficiencies were variable depending on the original inoculum but were consistently over 50% when 104 to 105 parasites were added. The use of this technique for evaluation of an antitrypanosomal agent, nifurtimox, was demonstrated, making possible large-scale testing of potential antitrypanosomal agents and assessment of microbicidal and microbio-static drug levels.",

keywords = "Agar medium, Chagas' disease, Cloning, Colony formation, Drug screening, Hemoflagellate, Nifurtimox, Protozoa, Trypanosoma cruzi, parasitic",

author = "Murray Wittner and Linda Squillante and Nadler, {Jeffrey P.} and Tanowitz, {Herbert B.}",

note = "Funding Information: This work was supported by Grant AI 12770 and Training Grant AI 07183 from the U.S. National Institutes of Health. The authors wish to thank Drs. Stephen Baum, E. Richard Stanley, and Michael Bir-rer for the critical review of the manuscript and discussions during the studies.",

year = "1982",

month = apr,

doi = "10.1016/0014-4894(82)90067-4",

language = "English (US)",

volume = "53",

pages = "255--261",

journal = "Experimental Parasitology",

issn = "0014-4894",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Trypanosoma cruzi

T2 - Colony formation and clonal growth in agar

AU - Wittner, Murray

AU - Squillante, Linda

AU - Nadler, Jeffrey P.

AU - Tanowitz, Herbert B.

N1 - Funding Information: This work was supported by Grant AI 12770 and Training Grant AI 07183 from the U.S. National Institutes of Health. The authors wish to thank Drs. Stephen Baum, E. Richard Stanley, and Michael Bir-rer for the critical review of the manuscript and discussions during the studies.

PY - 1982/4

Y1 - 1982/4

N2 - Trypanosoma cruzi exhibited colonial growth when incorporated into 0.5-0.6% agar. Colonies were established from a single organism and clones readily derived. The plating efficiencies were variable depending on the original inoculum but were consistently over 50% when 104 to 105 parasites were added. The use of this technique for evaluation of an antitrypanosomal agent, nifurtimox, was demonstrated, making possible large-scale testing of potential antitrypanosomal agents and assessment of microbicidal and microbio-static drug levels.

AB - Trypanosoma cruzi exhibited colonial growth when incorporated into 0.5-0.6% agar. Colonies were established from a single organism and clones readily derived. The plating efficiencies were variable depending on the original inoculum but were consistently over 50% when 104 to 105 parasites were added. The use of this technique for evaluation of an antitrypanosomal agent, nifurtimox, was demonstrated, making possible large-scale testing of potential antitrypanosomal agents and assessment of microbicidal and microbio-static drug levels.

KW - Agar medium

KW - Chagas' disease

KW - Cloning

KW - Colony formation

KW - Drug screening

KW - Hemoflagellate

KW - Nifurtimox

KW - Protozoa

KW - Trypanosoma cruzi

KW - parasitic

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DO - 10.1016/0014-4894(82)90067-4

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AN - SCOPUS:0019985640

SN - 0014-4894

VL - 53

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JO - Experimental Parasitology

JF - Experimental Parasitology

IS - 2

ER -

Trypanosoma cruzi: Colony formation and clonal growth in agar

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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