Abstract
Several mouse in vivo neuronal recording techniques require head fixation. Head-fixed treadmill walking can be used to design tasks that enable the study of neural activity in the context of behavior. Here, we provide a detailed protocol for constructing a treadmill with tactile spatial cues, training mice on a rewarded behavioral task, and analyzing behavioral data. We discuss common problems and solutions we have developed to optimize training. Finally, we demonstrate how to test spatial memory performance using this task.
Original language | English (US) |
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Article number | 100770 |
Journal | STAR Protocols |
Volume | 2 |
Issue number | 3 |
DOIs | |
State | Published - Sep 17 2021 |
Keywords
- Behavior
- Microscopy
- Model Organisms
- Neuroscience
ASJC Scopus subject areas
- General Neuroscience
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology