Transplantation of pulmonary valve using a mouse model of heterotopic heart transplantation

Yong Ung Lee, Tai Yi, Iyore James, Shuhei Tara, Alexander J. Stuber, Kejal V. Shah, Avione Y. Lee, Tadahisa Sugiura, Narutoshi Hibino, Toshiharu Shinoka, Christopher K. Breuer

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed results. However, the cellular and molecular mechanisms of the neotissue development, valve thickening, and stenosis development are not researched extensively. To answer the above questions, we developed a murine heterotopic heart valve transplantation model. A heart valve was harvested from a valve donor mouse and transplanted to a heart donor mouse. The heart with a new valve was transplanted heterotopically to a recipient mouse. The transplanted heart showed its own heartbeat, independent of the recipient's heartbeat. The blood flow was quantified using a high frequency ultrasound system with a pulsed wave Doppler. The flow through the implanted pulmonary valve showed forward flow with minimal regurgitation and the peak flow was close to 100 mm/sec. This murine model of heart valve transplantation is highly versatile, so it can be modified and adapted to provide different hemodynamic environments and/or can be used with various transgenic mice to study neotissue development in a tissue engineered heart valve.

Original languageEnglish (US)
Article numbere51695
JournalJournal of Visualized Experiments
Issue number89
StatePublished - Jul 23 2014
Externally publishedYes


  • Congenital heart defect
  • Decellularized heart valve
  • Heterotopic heart transplantation
  • Issue 89
  • Medicine
  • Pulmonary valve
  • Tissue engineering
  • Transgenic mouse model

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


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