TY - JOUR
T1 - Translocation of the catalytic domain of diphtheria toxin across planar phospholipid bilayers by its own T domain
AU - Oh, Kyoung Joon
AU - Senzel, Lisa
AU - Collier, R. John
AU - Finkelstein, Alan
PY - 1999/7/20
Y1 - 1999/7/20
N2 - The T domain of diphtheria toxin is known to participate in the pH- dependent translocation of the catalytic C domain of the toxin across the endosomal membrane, but how it does so, and whether cellular proteins are also required for this process, remain unknown. Here, we report results showing that the T domain alone is capable of translocating the entire C domain across model, planar phospholipid bilayers in the absence of other proteins. The T domain therefore contains the entire molecular machinery for mediating transfer of the catalytic domain of diphtheria toxin across membranes.
AB - The T domain of diphtheria toxin is known to participate in the pH- dependent translocation of the catalytic C domain of the toxin across the endosomal membrane, but how it does so, and whether cellular proteins are also required for this process, remain unknown. Here, we report results showing that the T domain alone is capable of translocating the entire C domain across model, planar phospholipid bilayers in the absence of other proteins. The T domain therefore contains the entire molecular machinery for mediating transfer of the catalytic domain of diphtheria toxin across membranes.
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U2 - 10.1073/pnas.96.15.8467
DO - 10.1073/pnas.96.15.8467
M3 - Article
C2 - 10411898
AN - SCOPUS:0000483941
SN - 0027-8424
VL - 96
SP - 8467
EP - 8470
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 15
ER -