Transcutaneous glucose measurement using near-infrared spectroscopy during hypoglycemia

Ilan Gabriely, Robert Wozniak, Michèle Mevorach, Jonathan Kaplan, Yigal Aharon, Harry Shamoon

Research output: Contribution to journalArticlepeer-review

83 Scopus citations


OBJECTIVE - To analyze a transcutaneous near-infrared spectroscopy system as a technique for in vivo noninvasive blood glucose monitoring during euglycemia and hypoglycemia. RESEARCH DESIGN AND METHODS - Ten nondiabetic subjects and two patients with type 1 diabetes were examined in a total of 27 studies. In each study, the subject's plasma glucose was lowered to a hypoglycemic level (~55 mg/dl) followed by recovery to a glycemic level of ~115 mg/dl using an intravenous infusion of insulin and 20% dextrose. Plasma glucose levels were determined at 5-min intervals by standard glucose oxidase method and simultaneously by a near-infrared spectroscopic system. The plasma glucose measured by the standard method was used to create a calibration model that could predict glucose levels from the near-infrared spectral data. The two data sets were correlated during the decline and recovery in plasma glucose, within 10 mg/dl plasma glucose ranges, and were examined using the Clarke Error Grid Analysis. RESULTS - Two sets of 1,704 plasma glucose determinations were examined. The near-infrared predictions during the fall and recovery in plasma glucose were highly correlated (r = 0.96 and 0.95, respectively). When analyzed during 10 mg/dl plasma glucose segments, the mean absolute difference between the near-infrared spectroscopy method and the chemometric reference ranged from 3.3 to 4.4 mg/dl in the nondiabetic subjects and from 2.6 to 3.8 mg/dl in the patients with type 1 diabetes. Using the Error Grid Analysis, 97.7% of all the near-infrared predictions were assigned to the A-zone CONCLUSIONS - Our findings suggest that the near- infrared spectroscopy method can accurately predict plasma glucose levels during euglycemia and hypoglycemia in humans.

Original languageEnglish (US)
Pages (from-to)2026-2032
Number of pages7
JournalDiabetes care
Issue number12
StatePublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Advanced and Specialized Nursing


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