TY - JOUR
T1 - Transcriptome profile of the murine macrophage cell response to Candida parapsilosis
AU - Németh, Tibor
AU - Tóth, Adél
AU - Hamari, Zsuzsanna
AU - Falus, András
AU - Éder, Katalin
AU - Vágvölgyi, Csaba
AU - Guimaraes, Allan J.
AU - Nosanchuk, Joshua D.
AU - Gácser, Attila
N1 - Funding Information:
JDN is supported in part by an Irma T. Hirschl/Monique Weill-Caulier Trust Research Award. AG is supported by OTKA NN100374, NF84006 and by EMBO Installation Grant 1813. AG supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences. This research was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP 4.2.4. A/2-11-1-2012-0001 ‘National Excellence Program’.
PY - 2014/4
Y1 - 2014/4
N2 - Candida parapsilosis is a human fungal pathogen with increasing global significance. Understanding how macrophages respond to C. parapsilosis at the molecular level will facilitate the development of novel therapeutic paradigms. The complex response of murine macrophages to infection with C. parapsilosis was investigated at the level of gene expression using an Agilent mouse microarray. We identified 155 and 511 differentially regulated genes at 3 and 8. h post-infection, respectively. Most of the upregulated genes encoded molecules involved in immune response and inflammation, transcription, signaling, apoptosis, cell cycle, electron transport and cell adhesion. Typical of the classically activated macrophages, there was significant upregulation of genes coordinating the production of inflammatory cytokines such as TNF, IL-1 and IL-15. Further, we used both primary murine macrophages and macrophages differentiated from human peripheral mononuclear cells to confirm the upregulation of the TNF-receptor family member TNFRSF9 that is associated with Th1 T-helper cell responses. Additionally, the microarray data indicate significant differences between the response to C. parapsilosis infection and that of C. albicans.
AB - Candida parapsilosis is a human fungal pathogen with increasing global significance. Understanding how macrophages respond to C. parapsilosis at the molecular level will facilitate the development of novel therapeutic paradigms. The complex response of murine macrophages to infection with C. parapsilosis was investigated at the level of gene expression using an Agilent mouse microarray. We identified 155 and 511 differentially regulated genes at 3 and 8. h post-infection, respectively. Most of the upregulated genes encoded molecules involved in immune response and inflammation, transcription, signaling, apoptosis, cell cycle, electron transport and cell adhesion. Typical of the classically activated macrophages, there was significant upregulation of genes coordinating the production of inflammatory cytokines such as TNF, IL-1 and IL-15. Further, we used both primary murine macrophages and macrophages differentiated from human peripheral mononuclear cells to confirm the upregulation of the TNF-receptor family member TNFRSF9 that is associated with Th1 T-helper cell responses. Additionally, the microarray data indicate significant differences between the response to C. parapsilosis infection and that of C. albicans.
KW - Candida parapsilosis
KW - Host response
KW - Infection
KW - Macrophages
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U2 - 10.1016/j.fgb.2014.01.006
DO - 10.1016/j.fgb.2014.01.006
M3 - Article
C2 - 24530442
AN - SCOPUS:84896118623
SN - 1087-1845
VL - 65
SP - 48
EP - 56
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
ER -