Transcranial imaging of functional cerebral hemodynamic changes in single blood vessels using in vivo photoacoustic microscopy

Lun De Liao, Chin Teng Lin, Yen Yu I. Shih, Timothy Q. Duong, Hsin Yi Lai, Po Hsun Wang, Robby Wu, Siny Tsang, Jyh Yeong Chang, Meng Lin Li, You Yin Chen

Research output: Contribution to journalArticlepeer-review

75 Scopus citations


Optical imaging of changes in total hemoglobin concentration (HbT), cerebral blood volume (CBV), and hemoglobin oxygen saturation (SO 2) provides a means to investigate brain hemodynamic regulation. However, high-resolution transcranial imaging remains challenging. In this study, we applied a novel functional photoacoustic microscopy technique to probe the responses of single cortical vessels to left forepaw electrical stimulation in mice with intact skulls. Functional changes in HbT, CBV, and SO 2 in the superior sagittal sinus and different-sized arterioles from the anterior cerebral artery system were bilaterally imaged with unambiguous 36 × 65-μm 2 spatial resolution. In addition, an early decrease of SO 2 in single blood vessels during activation (i.e., 'the initial dip') was observed. Our results indicate that the initial dip occurred specifically in small arterioles of activated regions but not in large veins. This technique complements other existing imaging approaches for the investigation of the hemodynamic responses in single cerebral blood vessels.

Original languageEnglish (US)
Pages (from-to)938-951
Number of pages14
JournalJournal of Cerebral Blood Flow and Metabolism
Issue number6
StatePublished - Jun 2012
Externally publishedYes


  • cerebral blood volume
  • forepaw electrical stimulation
  • hemodynamic response
  • hemoglobin oxygen saturation
  • total hemoglobin concentration
  • transcranial photoacoustic microscopy

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine


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