The use of fluorescent proteins for intravital imaging of cancer cell invasion

James Hulit; Dmitriy Kedrin; Bojana Gligorijevic; David Entenberg; Jeffrey Wyckoff; John Condeelis; Jeffrey E. Segall

doi:10.1007/978-1-61779-797-2_2

The use of fluorescent proteins for intravital imaging of cancer cell invasion

James Hulit, Dmitriy Kedrin, Bojana Gligorijevic, David Entenberg, Jeffrey Wyckoff, John Condeelis, Jeffrey E. Segall

Research output: Chapter in Book/Report/Conference proceeding › Chapter

7 Scopus citations

Abstract

The analysis of cancer cell behavior in the primary tumor in living animals provides an opportunity to explore the process of invasion and intravasation in the complex microenvironment that is present in vivo. In this chapter, we describe the methods that we have developed for performing intravital imaging of mammary tumors. We provide procedures for generating tumors through injection of tumor cell lines, and multiphoton imaging using a skin-flap tumor dissection and a mammary imaging window.

Original language	English (US)
Title of host publication	In Vivo Cellular Imaging Using Fluorescent Proteins
Subtitle of host publication	Methods and Protocols
Editors	Robert M. Hoffman
Pages	15-30
Number of pages	16
DOIs	https://doi.org/10.1007/978-1-61779-797-2_2
State	Published - 2012

Publication series

Name	Methods in Molecular Biology
Volume	872
ISSN (Print)	1064-3745

Keywords

Intravital imaging
Live animal imaging
Multiphoton imaging

ASJC Scopus subject areas

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-61779-797-2_2

Cite this

Hulit, J., Kedrin, D., Gligorijevic, B., Entenberg, D., Wyckoff, J., Condeelis, J., & Segall, J. E. (2012). The use of fluorescent proteins for intravital imaging of cancer cell invasion. In R. M. Hoffman (Ed.), In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols (pp. 15-30). (Methods in Molecular Biology; Vol. 872). https://doi.org/10.1007/978-1-61779-797-2_2

The use of fluorescent proteins for intravital imaging of cancer cell invasion. / Hulit, James; Kedrin, Dmitriy; Gligorijevic, Bojana et al.
In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols. ed. / Robert M. Hoffman. 2012. p. 15-30 (Methods in Molecular Biology; Vol. 872).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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AU - Segall, Jeffrey E.

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The use of fluorescent proteins for intravital imaging of cancer cell invasion

Abstract

Publication series

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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