@article{5c95ffd4c21d4caba80cfe6ebecab1dc,
title = "The transcription factor Pax6 regulates survival of dopaminergic olfactory bulb neurons via crystallin αA",
abstract = "Most neurons in the adult mammalian brain survive for the entire life of an individual. However, it is not known which transcriptional pathways regulate this survival in a healthy brain. Here, we identify a pathway regulating neuronal survival in a highly subtype-specific manner. We show that the transcription factor Pax6 expressed in dopaminergic neurons of the olfactory bulb regulates the survival of these neurons by directly controlling the expression of crystallin αA (CryαA), which blocks apoptosis by inhibition of procaspase-3 activation. Re-expression of CryαA fully rescues survival of Pax6-deficient dopaminergic interneurons in vivo and knockdown of CryαA by shRNA in wild-type mice reduces the number of dopaminergic OB interneurons. Strikingly, Pax6 utilizes different DNA-binding domains for its well-known role in fate specification and this role of regulating the survival of specific neuronal subtypes in the mature, healthy brain.",
author = "Jovica Ninkovic and Luisa Pinto and Stefania Petricca and Alexandra Lepier and Jian Sun and Rieger, {Michael A.} and Timm Schroeder and Ales Cvekl and Jack Favor and Magdalena G{\"o}tz",
note = "Funding Information: We would like to thank foremost Ruth Ashery-Padan for Pax6 fl/fl animals and Jochen Graw for his advice on crystallin function and in situ probes. We thank Yves Barde and Michael Sendtner for their input on the concepts of cell death in the CNS and the analysis of the programmed cell death pathway. We would also like to greatly acknowledge the excellent technical expertise of Andrea Steiner-Mezzadri, Emily Baumgart-Violette, Angelika Waiser, and Detelf Franzen. Many thanks also to Yves Barde, Emily Baumgart-Violette, Benedikt Berninger, and Armen Saghatelyan for great comments on the manuscript. The work was supported by the DFG including the SFB 596 and 870, the BMBF, the Bavarian Ministery for Science and Research (For NeuroCell), the Helmholtz Association (Helma), and the EU (EUTRACC). T.S. was partially supported by the DFG, and A.C. is supported by NIH grants EY012200 and EY014237. ",
year = "2010",
month = nov,
day = "18",
doi = "10.1016/j.neuron.2010.09.030",
language = "English (US)",
volume = "68",
pages = "682--694",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "4",
}