The Ski protein can inhibit ligand induced RARα and HDAC3 degradation in the retinoic acid signaling pathway

Hong Ling Zhao; Nobuhide Ueki; Katherine Marcelain; Michael J. Hayman

doi:10.1016/j.bbrc.2009.03.141

The Ski protein can inhibit ligand induced RARα and HDAC3 degradation in the retinoic acid signaling pathway

Hong Ling Zhao, Nobuhide Ueki, Katherine Marcelain, Michael J. Hayman

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Recent data has implicated the Ski protein as being a physiologically relevant negative regulator of signaling by retinoic acid (RA). The mechanism by which Ski represses RA signaling is unknown. Co-immunoprecipitation and immunofluorescence assay showed that Ski and RARα are in the same complex in both the absence and presence of RA, which makes Ski different from other corepressors. We determined that Ski can stabilize RARα and HDAC3. These results suggest that Ski represses RA signaling by stabilizing corepressor complex.

Original language	English (US)
Pages (from-to)	119-124
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	383
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2009.03.141
State	Published - May 22 2009
Externally published	Yes

Keywords

HDAC3
Nuclear hormone signaling
Proteasome
RA
RARα
Ski

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2009.03.141

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title = "The Ski protein can inhibit ligand induced RARα and HDAC3 degradation in the retinoic acid signaling pathway",

abstract = "Recent data has implicated the Ski protein as being a physiologically relevant negative regulator of signaling by retinoic acid (RA). The mechanism by which Ski represses RA signaling is unknown. Co-immunoprecipitation and immunofluorescence assay showed that Ski and RARα are in the same complex in both the absence and presence of RA, which makes Ski different from other corepressors. We determined that Ski can stabilize RARα and HDAC3. These results suggest that Ski represses RA signaling by stabilizing corepressor complex.",

keywords = "HDAC3, Nuclear hormone signaling, Proteasome, RA, RARα, Ski",

author = "Zhao, {Hong Ling} and Nobuhide Ueki and Katherine Marcelain and Hayman, {Michael J.}",

note = "Funding Information: We thank all the members of the Hayman laboratory for helpful discussions and criticisms of the manuscript. This work was supported by U.S. Public Health Service Grant CA42573 from the National Cancer Institute to M.J.H.",

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T1 - The Ski protein can inhibit ligand induced RARα and HDAC3 degradation in the retinoic acid signaling pathway

AU - Zhao, Hong Ling

AU - Ueki, Nobuhide

AU - Marcelain, Katherine

AU - Hayman, Michael J.

N1 - Funding Information: We thank all the members of the Hayman laboratory for helpful discussions and criticisms of the manuscript. This work was supported by U.S. Public Health Service Grant CA42573 from the National Cancer Institute to M.J.H.

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N2 - Recent data has implicated the Ski protein as being a physiologically relevant negative regulator of signaling by retinoic acid (RA). The mechanism by which Ski represses RA signaling is unknown. Co-immunoprecipitation and immunofluorescence assay showed that Ski and RARα are in the same complex in both the absence and presence of RA, which makes Ski different from other corepressors. We determined that Ski can stabilize RARα and HDAC3. These results suggest that Ski represses RA signaling by stabilizing corepressor complex.

AB - Recent data has implicated the Ski protein as being a physiologically relevant negative regulator of signaling by retinoic acid (RA). The mechanism by which Ski represses RA signaling is unknown. Co-immunoprecipitation and immunofluorescence assay showed that Ski and RARα are in the same complex in both the absence and presence of RA, which makes Ski different from other corepressors. We determined that Ski can stabilize RARα and HDAC3. These results suggest that Ski represses RA signaling by stabilizing corepressor complex.

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KW - Nuclear hormone signaling

KW - Proteasome

KW - RA

KW - RARα

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The Ski protein can inhibit ligand induced RARα and HDAC3 degradation in the retinoic acid signaling pathway

Abstract

Keywords

ASJC Scopus subject areas

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