TY - JOUR
T1 - The role of speckle tracking echocardiography in assessment of lipopolysaccharide-induced myocardial dysfunction in mice
AU - Chu, Ming
AU - Gao, Yao
AU - Zhang, Yanjuan
AU - Zhou, Bin
AU - Wu, Bingruo
AU - Yao, Jing
AU - Xu, Di
N1 - Funding Information:
We thank Wendy Lui, PHD in Departments of Genetics, Pediatrics and Medicine (Cardiology), Albert Einstein College of Medicine of Yeshiva University, for her critical revising of the manuscript for English language and grammar. Funding: This work was supported by the National Natural Science Foundation of China (NSFC 81000618) to Dr. Yao Jing, (NSFC 81271589) to Prof. Xu Di and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Funding Information:
We thank Wendy Lui, PHD in Departments of Genetics, Pediatrics and Medicine (Cardiology), Albert Einstein College of Medicine of Yeshiva University, for her critical revising of the manuscript for English language and grammar. Funding: This work was supported by the National Natural Science Foundation of China (NSFC 81000618) to Dr. Yao Jing, (NSFC 81271589) to Prof. Xu Di and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Publisher Copyright:
© Journal of Thoracic Disease.
PY - 2015
Y1 - 2015
N2 - Background: Sepsis-induced myocardial dysfunction is a common and severe complication of septic shock. Conventional echocardiography often fails to reveal myocardial depression in severe sepsis due to hemodynamic changes; in contrast, decline of strain measurements by speckle tracking echocardiography (STE) may indicate impaired cardiac function. This study investigates the role of STE in detecting lipopolysaccharide (LPS)-induced cardiac dysfunction with mouse models. Methods: We evaluated cardiac function in 20 mice at baseline, 6 h (n=10) and 20 h ( n=10) after LPS injection to monitor the development of heart failure induced by severe sepsis using 2-D and M-mode echocardiography. Ejection fraction (EF) and fractional shortening (FS) were measured with standard M-mode tracings, whereas circumferential and radial strain was derived from STE. Serum biochemical and cardiac histopathological examinations were performed to determine sepsis-induced myocardial injury. Results: Left ventricular (LV) myocardial function was significantly reduced at 6 h after LPS treatment assessed by circumferential strain (-14.65%±3.00% to -8.48%±1.72%, P=0.006), whereas there were no significant differences between 6 and 20 h group. Conversely, EF and FS were significantly increased at 20 h when comparing to 6 h (P < 0.05) accompanied with marked decreases in EF and FS 6 h following LPS administration. Consistent with strain echocardiographic results, we showed that LPS injection leaded to elevated serum level of cardiac Troponin-T (cTnT), CK-MB and rising leucocytes infiltration into myocardium within 20 h. Conclusions: Altogether, these results demonstrate that, circumferential strain by STE is a specific and reliable value for evaluating LPS-induced cardiac dysfunction in mice.
AB - Background: Sepsis-induced myocardial dysfunction is a common and severe complication of septic shock. Conventional echocardiography often fails to reveal myocardial depression in severe sepsis due to hemodynamic changes; in contrast, decline of strain measurements by speckle tracking echocardiography (STE) may indicate impaired cardiac function. This study investigates the role of STE in detecting lipopolysaccharide (LPS)-induced cardiac dysfunction with mouse models. Methods: We evaluated cardiac function in 20 mice at baseline, 6 h (n=10) and 20 h ( n=10) after LPS injection to monitor the development of heart failure induced by severe sepsis using 2-D and M-mode echocardiography. Ejection fraction (EF) and fractional shortening (FS) were measured with standard M-mode tracings, whereas circumferential and radial strain was derived from STE. Serum biochemical and cardiac histopathological examinations were performed to determine sepsis-induced myocardial injury. Results: Left ventricular (LV) myocardial function was significantly reduced at 6 h after LPS treatment assessed by circumferential strain (-14.65%±3.00% to -8.48%±1.72%, P=0.006), whereas there were no significant differences between 6 and 20 h group. Conversely, EF and FS were significantly increased at 20 h when comparing to 6 h (P < 0.05) accompanied with marked decreases in EF and FS 6 h following LPS administration. Consistent with strain echocardiographic results, we showed that LPS injection leaded to elevated serum level of cardiac Troponin-T (cTnT), CK-MB and rising leucocytes infiltration into myocardium within 20 h. Conclusions: Altogether, these results demonstrate that, circumferential strain by STE is a specific and reliable value for evaluating LPS-induced cardiac dysfunction in mice.
KW - Endotoxin-induced cardiac dysfunction
KW - Sepsis
KW - Septic cardiomyopathy
KW - Speckle tracking echocardiography (STE)
KW - Strain imaging
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U2 - 10.3978/j.issn.2072-1439.2015.12.37
DO - 10.3978/j.issn.2072-1439.2015.12.37
M3 - Article
AN - SCOPUS:84954176348
SN - 2072-1439
VL - 7
SP - 2253
EP - 2261
JO - Journal of Thoracic Disease
JF - Journal of Thoracic Disease
IS - 12
ER -