@article{872ff04db4af4e08b4ed4453f6abe91f,
title = "The identification of monoclonal class switch variants by sib selection and an ELISA assay",
abstract = "Monoclonal antibodies can now be generated against a wide variety of antigens. However, a potentially useful monoclonal antibody may be of the wrong class or subclass for a particular task. Antibodies of the desired class can be obtained by identifying rare subclones in which the variable region has been rearranged to a new constant region. We describe here the use of sib selection and an enzyme-linked immunoassay (ELISA) for isolating such class and subclass switch variants from 2 IgM and 1 IgG3 producing hybridoma. The relative simplicity of ELISA assays make it feasible to apply this approach to many different hybridomas. Since commercial affinity purified class and subclass specific antibodies are now available, the method can be used by any laboratory. Furthermore, the technique does not require that the switch variants express surface immunoglobulin and enriches for hybridomas secreting higher amounts of antibody.",
keywords = "ELISA, cell line, class and subclass switch, monoclonal antibody, sib selection",
author = "Gad Spira and Antonio Bargellesi and Teillaud, {Jean Luc} and Scharff, {Matthew D.}",
note = "Funding Information: The potential of making monoclonal antibodies specific for a wide variety of antigens has led to their use in serology in many areas of biology and medicine. If large numbers of monoclonals can be produced with the desired specificity and affinity, it is sometimes possible to choose antibodies of different classes and subclasses for tasks which require specific effector functions. However, this is often not possible because the antibodies elicited by some antigens are predominantly of 1 1 Present address: Faculty of Medicine, Technion, Haifa, Israel. 2 Present address: I Facolth di Chimica Biologica, University of Genoa, Genoa, Italy. 3 Present address: lnstitut Curie, Laboratoire d'lmmunologie Clinique, 26 rue d'Ulm, 75005 Paris, France. Supported by the Cancer Research Institute of the City of New York and the French Ministry of Research and Industry. 4 To whom correspondence should be addressed. Funding Information: This work was supported by NIH Grants (AI10702, AI5231); NSF Grant number PCM81-08642 and ACSIM317C. Partial support was provided by an NCI Core Cancer Grant number NIH/NCI P30 CA13330-12 and the Atran Foundation.",
year = "1984",
month = nov,
day = "30",
doi = "10.1016/0022-1759(84)90298-9",
language = "English (US)",
volume = "74",
pages = "307--315",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "2",
}