The herpes simplex virus 1 US3 regulates phospholipid synthesis

Peter Wild, Anna Paula De Oliveira, Sabrina Sonda, Elisabeth M. Schraner, Mathias Ackermann, Kurt Tobler

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Herpes simplex virus type 1 capsids bud at nuclear and Golgi membranes for envelopment by phospholipid bilayers. In the absence of US3, nuclear membranes form multiple folds harboring virions that suggests disturbance in membrane turnover. Therefore, we investigated phospholipid metabolism in cells infected with the US3 deletion mutant R7041(ΔUS3), and quantified membranes involved in viral envelopment. We report that (i) [3H]-choline incorporation into nuclear membranes and cytoplasmic membranes was enhanced peaking at 12 or 20h post inoculation with wild type HSV-1 and R7041(ΔUS3), respectively, (ii) the surface area of nuclear membranes increased until 24h of R7041(ΔUS3) infection forming folds that equaled ~45% of the nuclear surface, (iii) the surface area of viral envelopes between nuclear membranes equaled ~2400 R7041(ΔUS3) virions per cell, and (iv) during R7041(ΔUS3) infection, the Golgi complex expanded dramatically. The data indicate that US3 plays a significant role in regulation of membrane biosynthesis.

Original languageEnglish (US)
Pages (from-to)353-360
Number of pages8
Issue number2
StatePublished - Oct 25 2012
Externally publishedYes


  • Golgi membranes
  • Herpes virus
  • Morphometry
  • Nuclear membranes
  • Phospholipid synthesis
  • TEM
  • [H]-choline

ASJC Scopus subject areas

  • Virology


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