TY - JOUR
T1 - The hemoglobin of ascaris body walls
AU - Okazaki, Taro
AU - Wittenberg, Beatrice A.
AU - Briehl, R. W.
AU - Wittenberg, Jonathan B.
N1 - Funding Information:
We thank Drs. EMMETT W. BASSETT and STUART TANENBAUM, Department of Microbiology, Columbia University, for the amino acid analysis. It is a pleasure to acknowledge the assistance of Mrs. LIDIJA KAMPA. This investigation was supported in part by a Public Service Research Grant (HE-o3719) from the National Heart Institute and in part by a Research Grant from the National Science Foundation (G2oo2o).
Funding Information:
1-232). * Career Scientist of the Health Research Council of the City of New York (contract number
PY - 1967/6/27
Y1 - 1967/6/27
N2 - The hemoglobin of the body wall of the parasitic nematode, Ascaris lumbricoides, L., has been purified. The sedimentation coefficient, s20,w is 3.1 S. The molecular weight, deduced from sedimentation equilibrium by the method of Yphantis, is 37 000. The molecular weight per heme group, deduced from amino acid analysis, is 35 000, and there is therefore 1 heme group per molecule. The amino acid composition of the body-wall hemoglobin differs from that of the hemoglobin found in the perienteric fluid. The absorption spectrum of the hemoglobin and several of its derivatives are presented. In general they resemble those of perienteric fluid hemoglobin although many maxima are shifted about 2 mμ toward the red. The ferrous derivatives each display maxima at 614-624 mμ, which are not artifacts since they may be seen in the surviving intact body wall.
AB - The hemoglobin of the body wall of the parasitic nematode, Ascaris lumbricoides, L., has been purified. The sedimentation coefficient, s20,w is 3.1 S. The molecular weight, deduced from sedimentation equilibrium by the method of Yphantis, is 37 000. The molecular weight per heme group, deduced from amino acid analysis, is 35 000, and there is therefore 1 heme group per molecule. The amino acid composition of the body-wall hemoglobin differs from that of the hemoglobin found in the perienteric fluid. The absorption spectrum of the hemoglobin and several of its derivatives are presented. In general they resemble those of perienteric fluid hemoglobin although many maxima are shifted about 2 mμ toward the red. The ferrous derivatives each display maxima at 614-624 mμ, which are not artifacts since they may be seen in the surviving intact body wall.
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U2 - 10.1016/0005-2795(67)90466-7
DO - 10.1016/0005-2795(67)90466-7
M3 - Article
C2 - 6048305
AN - SCOPUS:0014218569
SN - 0005-2795
VL - 140
SP - 258
EP - 265
JO - BBA - Protein Structure
JF - BBA - Protein Structure
IS - 2
ER -