TY - JOUR
T1 - The effect of silver nanoparticles on antioxidant/pro-oxidant balance in a murine model
AU - Docea, Anca Oana
AU - Calina, Daniela
AU - Buga, Ana Maria
AU - Zlatian, Ovidiu
AU - Paoliello, M. M.B.
AU - Mogosanu, George Dan
AU - Streba, Costin Teodor
AU - Popescu, Elena Leocadia
AU - Stoica, Alexandra Elena
AU - Bîrcă, Alexandra Catalina
AU - Vasile, Bogdan Ștefan
AU - Grumezescu, Alexandru Mihai
AU - Mogoanta, Laurentiu
N1 - Funding Information:
Author Contributions: A.O.D., D.C., A.M.B., O.Z. contributed equally in writing the manuscript and substantially contributed to study design, data acquisition, including data analysis and interpretation. A.O.D., D.C., M.M.B.P. and L.M. coordinated conception of the study. O.Z., G.D.M., C.T.S. and E.L.P. used statistical software for the data interpretation. A.E.S., A.C.B., B.S.V. and A.M.G. contributed to writing the manuscript. A.O.D., D.C. and L.M. supervised the whole process. All authors have read and agreed to the published version of the manuscript.FundingThis work was funded by a grant from the Romanian National Authority for Scientific Research and Innovation, UEFISCDI, project number 45PCCDI/2018–“Bioactive nanostructures for innovative therapeutic strategies”.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/2
Y1 - 2020/2
N2 - This study aimed to evaluate the subacute effect of two types of Ag-NPs(EG-AgNPs and PVP-EG-AgNPs) on antioxidant/pro-oxidant balance in rats. Seventy Wistar rats (35 males and 35 females) were divided in 7 groups and intraperitoneally exposed for 28 days to 0, 1, 2 and 4 mg/kg bw/day EG-Ag-NPs and 1, 2 and 4 mg/kg bw/day PVP-EG-Ag-NPs. After 28 days, the blood was collected, and the total antioxidant capacity (TAC), thiobarbituric reactive species (TBARS),protein carbonyl (PROTC) levels, reduced glutathione (GSH) levels and catalase (CAT) activity were determined. EG-Ag-NPs determined protective antioxidant effects in a dose-dependent manner. The exposure to the 4 mg/kg bw/day EG-Ag-NPs determines both in males and females a significant increase in TAC and CAT and a significant decrease in TBARS and PROTC only in females. The PVP-EG-AgNPs showed a different trend compared to EG-AgNPs. At 4 mg/kg bw/day the PVP-EG-AgNPs induce increased PROTC levels and decreased GSH (males and females) and TAC levels (males). The different mechanisms of EG-AgNPs and PVP-EG-AgNPs on antioxidant-/pro-oxidant balance can be explained by the influence of coating agent used for the preparation of the nanoparticles in the formation and composition of protein corona that influence their pathophysiology in the organism.
AB - This study aimed to evaluate the subacute effect of two types of Ag-NPs(EG-AgNPs and PVP-EG-AgNPs) on antioxidant/pro-oxidant balance in rats. Seventy Wistar rats (35 males and 35 females) were divided in 7 groups and intraperitoneally exposed for 28 days to 0, 1, 2 and 4 mg/kg bw/day EG-Ag-NPs and 1, 2 and 4 mg/kg bw/day PVP-EG-Ag-NPs. After 28 days, the blood was collected, and the total antioxidant capacity (TAC), thiobarbituric reactive species (TBARS),protein carbonyl (PROTC) levels, reduced glutathione (GSH) levels and catalase (CAT) activity were determined. EG-Ag-NPs determined protective antioxidant effects in a dose-dependent manner. The exposure to the 4 mg/kg bw/day EG-Ag-NPs determines both in males and females a significant increase in TAC and CAT and a significant decrease in TBARS and PROTC only in females. The PVP-EG-AgNPs showed a different trend compared to EG-AgNPs. At 4 mg/kg bw/day the PVP-EG-AgNPs induce increased PROTC levels and decreased GSH (males and females) and TAC levels (males). The different mechanisms of EG-AgNPs and PVP-EG-AgNPs on antioxidant-/pro-oxidant balance can be explained by the influence of coating agent used for the preparation of the nanoparticles in the formation and composition of protein corona that influence their pathophysiology in the organism.
KW - Antioxidant activity
KW - Oxidative stress
KW - Silver nanoparticles
KW - Subacute toxicity
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UR - http://www.scopus.com/inward/citedby.url?scp=85079545066&partnerID=8YFLogxK
U2 - 10.3390/ijms21041233
DO - 10.3390/ijms21041233
M3 - Article
C2 - 32059471
AN - SCOPUS:85079545066
SN - 1661-6596
VL - 21
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 4
M1 - 1233
ER -