The effect of silver nanoparticles on antioxidant/pro-oxidant balance in a murine model

Anca Oana Docea, Daniela Calina, Ana Maria Buga, Ovidiu Zlatian, M. M.B. Paoliello, George Dan Mogosanu, Costin Teodor Streba, Elena Leocadia Popescu, Alexandra Elena Stoica, Alexandra Catalina Bîrcă, Bogdan Ștefan Vasile, Alexandru Mihai Grumezescu, Laurentiu Mogoanta

Research output: Contribution to journalArticlepeer-review

69 Scopus citations


This study aimed to evaluate the subacute effect of two types of Ag-NPs(EG-AgNPs and PVP-EG-AgNPs) on antioxidant/pro-oxidant balance in rats. Seventy Wistar rats (35 males and 35 females) were divided in 7 groups and intraperitoneally exposed for 28 days to 0, 1, 2 and 4 mg/kg bw/day EG-Ag-NPs and 1, 2 and 4 mg/kg bw/day PVP-EG-Ag-NPs. After 28 days, the blood was collected, and the total antioxidant capacity (TAC), thiobarbituric reactive species (TBARS),protein carbonyl (PROTC) levels, reduced glutathione (GSH) levels and catalase (CAT) activity were determined. EG-Ag-NPs determined protective antioxidant effects in a dose-dependent manner. The exposure to the 4 mg/kg bw/day EG-Ag-NPs determines both in males and females a significant increase in TAC and CAT and a significant decrease in TBARS and PROTC only in females. The PVP-EG-AgNPs showed a different trend compared to EG-AgNPs. At 4 mg/kg bw/day the PVP-EG-AgNPs induce increased PROTC levels and decreased GSH (males and females) and TAC levels (males). The different mechanisms of EG-AgNPs and PVP-EG-AgNPs on antioxidant-/pro-oxidant balance can be explained by the influence of coating agent used for the preparation of the nanoparticles in the formation and composition of protein corona that influence their pathophysiology in the organism.

Original languageEnglish (US)
Article number1233
JournalInternational Journal of Molecular Sciences
Issue number4
StatePublished - Feb 2020


  • Antioxidant activity
  • Oxidative stress
  • Silver nanoparticles
  • Subacute toxicity

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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