Abstract
Background: careHPV is a new, lower-cost DNA test for human papillomavirus (HPV). There are limited analytic comparisons of careHPV against a referent HPV DNA test like Hybrid Capture 2 (HC2). Objective: To assess the test agreement between careHPV and HC2 on self- and clinician-collected specimens. Study design: In a population of 7541 women living in rural China, women provided a self-collected (sc) and two clinician-collected (cc) specimens and underwent visual inspection after acetic acid (VIA). The sc specimen and one cc specimen were tested by careHPV and HC2; a random subset of specimens was tested for HPV genotypes. Results: The percent positive on cc specimens and sc specimens was 14.69% and 14.97% for careHPV, respectively, and 15.05% and 18.53% for HC2, respectively; HC2 testing of sc specimens was more likely to test positive than other combinations of tests and specimens (p<0.0001 for all comparisons). The agreement between different tests on the same specimens (kappa=0.787 and 0.691 for cc and sc specimens, respectively) was better than the same test on different specimens (kappa=0.653 and 0.649 for HC2 and careHPV, respectively). Disagreement between the same test on different specimens increased with increasing participant age (ptrend=0.0001 for HC2 and 0.002 for careHPV). HC2-positive/careHPV-negative specimens were more likely to test positive for non-carcinogenic HPV genotype than test HPV negative whereas the converse was true for HC2-negative/careHPV-positive specimens. Discussion: The agreement for HPV DNA detection between careHPV and HC2 was good to very good.
Original language | English (US) |
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Pages (from-to) | 553-557 |
Number of pages | 5 |
Journal | Journal of Clinical Virology |
Volume | 61 |
Issue number | 4 |
DOIs | |
State | Published - 2014 |
Externally published | Yes |
Keywords
- Cervical cancer
- Cervical intraepithelial neoplasia (CIN)
- Human papillomavirus (HPV)
- Visual inspection after acetic acid (VIA)
ASJC Scopus subject areas
- Virology
- Infectious Diseases