TY - JOUR
T1 - The clinical and genomic significance of donor-specific antibody-positive/C4d-negative and donor-specific antibody-negative/C4d-negative transplant glomerulopathy
AU - Hayde, Nicole
AU - Bao, Yi
AU - Pullman, James
AU - Ye, Bin
AU - Brent Calder, R.
AU - Chung, Monica
AU - Schwartz, Daniel
AU - Lubetzky, Michelle
AU - Ajaimy, Maria
AU - de Boccardo, Graciela
AU - Akalin, Enver
PY - 2013/12/6
Y1 - 2013/12/6
N2 - Background This study investigated themechanisms involved in development of donor-specific antibody (DSA) and/or C4d-negative transplant glomerulopathy (TGP) by allograft gene expression profiles using microarrays. Design, Setting, Participants, & Measurements This cohort study was conducted in kidney transplant recipients. Patients were eligible for inclusion if they required a clinically indicated biopsy at any time point after their transplant. They were then classified according to their histopathology findings and DSA and C4d results. Eighteen chronic antibody-mediated rejection (CAMR), 14 DSA+/C4d2 TGP, 25 DSA2/C4d2 TGP, and 47 nonspecific interstitial fibrosis/tubular atrophy (IFTA) biopsy specimens were identified. In a subset of patients from the study population, biopsy specimens in each group and normal transplant kidney specimens were analyzed with Affymetrix Human Gene 1.0 ST Arrays. Results Themean sumscore of glomerulitis and peritubular capillaritis increased from 0.28±0.78 in IFTA specimens to 0.75±0.85 in DSA2/C4d2TGP specimens, 1.71±1.49 in DSA+/C4d2/TGP specimens, and 2.11±1.74 inCAMR specimens (P<0.001).During a median follow-up time of 2 (interquartile range, 1.4-2.8) years after biopsy, graft loss was highest in CAMR specimens (27.8%) compared to IFTA specimens (8.5%), DSA+/C4d2 TGP specimens (14.3%), and DSA2/C4d2 TGP specimens (16%) (P=0.01).With use of microarrays, comparison of the gene expression profiles of DSA2/C4d2 TGP specimens with glomerulitis + peritubular capillaritis scores > 0 to normal and IFTA biopsy specimens revealed higher expression of quantitative cytotoxic T cell-associated transcripts (QCAT).However, both CAMR and DSA+/C4d2TGP specimens had higher expression of not onlyQCATbut also IFN-γ and rejection-induced, constitutive macrophage-associated, natural killer cell-associated, and DSA-selective transcripts. Endothelial cell-associated transcript expression was upregulated only in CAMR biopsy specimens. Conclusions These results suggested that DSA+/C4d2 TGP biopsy specimens may be classified as CAMR. In contrast, DSA2/C4d2 TGP specimens showed increased cytotoxic T cell-associated transcripts, suggesting T cell activation as a mechanism of injury.
AB - Background This study investigated themechanisms involved in development of donor-specific antibody (DSA) and/or C4d-negative transplant glomerulopathy (TGP) by allograft gene expression profiles using microarrays. Design, Setting, Participants, & Measurements This cohort study was conducted in kidney transplant recipients. Patients were eligible for inclusion if they required a clinically indicated biopsy at any time point after their transplant. They were then classified according to their histopathology findings and DSA and C4d results. Eighteen chronic antibody-mediated rejection (CAMR), 14 DSA+/C4d2 TGP, 25 DSA2/C4d2 TGP, and 47 nonspecific interstitial fibrosis/tubular atrophy (IFTA) biopsy specimens were identified. In a subset of patients from the study population, biopsy specimens in each group and normal transplant kidney specimens were analyzed with Affymetrix Human Gene 1.0 ST Arrays. Results Themean sumscore of glomerulitis and peritubular capillaritis increased from 0.28±0.78 in IFTA specimens to 0.75±0.85 in DSA2/C4d2TGP specimens, 1.71±1.49 in DSA+/C4d2/TGP specimens, and 2.11±1.74 inCAMR specimens (P<0.001).During a median follow-up time of 2 (interquartile range, 1.4-2.8) years after biopsy, graft loss was highest in CAMR specimens (27.8%) compared to IFTA specimens (8.5%), DSA+/C4d2 TGP specimens (14.3%), and DSA2/C4d2 TGP specimens (16%) (P=0.01).With use of microarrays, comparison of the gene expression profiles of DSA2/C4d2 TGP specimens with glomerulitis + peritubular capillaritis scores > 0 to normal and IFTA biopsy specimens revealed higher expression of quantitative cytotoxic T cell-associated transcripts (QCAT).However, both CAMR and DSA+/C4d2TGP specimens had higher expression of not onlyQCATbut also IFN-γ and rejection-induced, constitutive macrophage-associated, natural killer cell-associated, and DSA-selective transcripts. Endothelial cell-associated transcript expression was upregulated only in CAMR biopsy specimens. Conclusions These results suggested that DSA+/C4d2 TGP biopsy specimens may be classified as CAMR. In contrast, DSA2/C4d2 TGP specimens showed increased cytotoxic T cell-associated transcripts, suggesting T cell activation as a mechanism of injury.
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U2 - 10.2215/CJN.04240413
DO - 10.2215/CJN.04240413
M3 - Article
C2 - 24030736
AN - SCOPUS:84889838399
SN - 1555-9041
VL - 8
SP - 2141
EP - 2148
JO - Clinical Journal of the American Society of Nephrology
JF - Clinical Journal of the American Society of Nephrology
IS - 12
ER -