TY - JOUR
T1 - The characteristics of peritoneal healing after catheter insertion in a rat model of peritoneal dialysis.
AU - Wieczorowska-Tobis, K.
AU - Polubinska, A.
AU - Kuzlan-Pawlaczyk, M.
AU - Pawlaczyk, K.
AU - Breborowicz, A.
AU - Oreopoulos, D. G.
PY - 1998
Y1 - 1998
N2 - The aim of this study was to characterize the process of peritoneal healing after catheter insertion in a rat model of peritoneal dialysis using a 1.36% solution (Baxter Dianeal). After catheter insertion (day 1 of experiment), 12 rats were injected daily with 20 mL of 1.36% dialysis solution for 2 weeks. Every second day a sample of 4 hour dialysate was taken from every rat for biochemical analysis, cell count, cell differentiation, and nitrites measurement. The measured parameters decreased during the experiment and stabilized during the second week after catheter insertion. In cell differentiation, we observed an increase in the number of macrophages, with a parallel decrease in the number of neutrophils that was reflected in a significant decrease in the neutrophils/macrophages ratio. Our results suggest that the process of peritoneal healing after catheter implantation in rats lasts about 2 weeks, and therefore that any biocompatibility study of peritoneal dialysis solutions in rats should be performed at least 2 weeks after catheter insertion.
AB - The aim of this study was to characterize the process of peritoneal healing after catheter insertion in a rat model of peritoneal dialysis using a 1.36% solution (Baxter Dianeal). After catheter insertion (day 1 of experiment), 12 rats were injected daily with 20 mL of 1.36% dialysis solution for 2 weeks. Every second day a sample of 4 hour dialysate was taken from every rat for biochemical analysis, cell count, cell differentiation, and nitrites measurement. The measured parameters decreased during the experiment and stabilized during the second week after catheter insertion. In cell differentiation, we observed an increase in the number of macrophages, with a parallel decrease in the number of neutrophils that was reflected in a significant decrease in the neutrophils/macrophages ratio. Our results suggest that the process of peritoneal healing after catheter implantation in rats lasts about 2 weeks, and therefore that any biocompatibility study of peritoneal dialysis solutions in rats should be performed at least 2 weeks after catheter insertion.
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M3 - Article
C2 - 10649687
AN - SCOPUS:0032222530
SN - 1197-8554
VL - 14
SP - 36
EP - 39
JO - Advances in peritoneal dialysis. Conference on Peritoneal Dialysis
JF - Advances in peritoneal dialysis. Conference on Peritoneal Dialysis
ER -