TY - JOUR
T1 - Taxol stabilizes microtubules in mouse fibroblast cells
AU - Schiff, P. B.
AU - Horwitz, S. B.
PY - 1980
Y1 - 1980
N2 - Taxol, a potent inhibitor of human HeLa and mouse fibroblast cell replication, blocked cells in the G2 and M phase of the cell cycle and stabilized cytoplasmic microtubules. The cytoplasmic microtubules of taxol-treated cells were visualized by transmission electron microscopy and indirect immunofluorescence microscopy. More than 90% of the cells treated with 10 μM taxol for 22 hr at 37°C displayed bundles of microtubules that appeared to radiate from a common site (or sites), in addition to their cytoplasmic microtubules. Untreated cells that were kept in the cold (4°C) for 16 hr lost their microtubules, whereas cells that were pretreated with taxol for 22 hr at 37°C continued to display their microtubules and bundles of microtubules in the cold. Taxol inhibited the migration behavior of fibroblast cells, but these cells did not lose their ability to produce mobile surface projections such as lamellipodia and filopodia.
AB - Taxol, a potent inhibitor of human HeLa and mouse fibroblast cell replication, blocked cells in the G2 and M phase of the cell cycle and stabilized cytoplasmic microtubules. The cytoplasmic microtubules of taxol-treated cells were visualized by transmission electron microscopy and indirect immunofluorescence microscopy. More than 90% of the cells treated with 10 μM taxol for 22 hr at 37°C displayed bundles of microtubules that appeared to radiate from a common site (or sites), in addition to their cytoplasmic microtubules. Untreated cells that were kept in the cold (4°C) for 16 hr lost their microtubules, whereas cells that were pretreated with taxol for 22 hr at 37°C continued to display their microtubules and bundles of microtubules in the cold. Taxol inhibited the migration behavior of fibroblast cells, but these cells did not lose their ability to produce mobile surface projections such as lamellipodia and filopodia.
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U2 - 10.1073/pnas.77.3.1561
DO - 10.1073/pnas.77.3.1561
M3 - Article
C2 - 6103535
AN - SCOPUS:0000297058
SN - 0027-8424
VL - 77
SP - 1561
EP - 1565
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 3 I
ER -