T-h-2 immunity and CD3+ CD45RBlow-activated T cells in mice immunized with recombinant bacillus Calmette-Guérin expressing HIV-1 principal neutralizing determinant epitope

Vivekanandhan Aravindhan; Sujatha Narayanan; Namasivayam Gautham; Vinayaka Prasad; P. Kannan; William R. Jacobs; Paranji R. Narayanan

doi:10.1111/j.1574-695X.2006.00061.x

T-h-2 immunity and CD3⁺ CD45RB^low-activated T cells in mice immunized with recombinant bacillus Calmette-Guérin expressing HIV-1 principal neutralizing determinant epitope

Vivekanandhan Aravindhan, Sujatha Narayanan, Namasivayam Gautham, Vinayaka Prasad, P. Kannan, William R. Jacobs, Paranji R. Narayanan

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The genetic engineering of Mycobacterium bovis-bacillus Calmette-Guérin to express foreign epitopes is an attractive strategy in the field of epitope vaccines. We constructed an 'epitope-trap vector' with Mycobacterium tuberculosis chaperonin-10 as a carrier antigen and used it to express the HIV-1 principal neutralizing determinant epitope. We also identified a new chaperonin-10 promoter that was hyperexpressive compared with the heat shock protein-65 promoter. Splenocytes from recombinant bacillus Calmette-Guérin-immunized mice showed enhanced lymphocyte proliferation and interleukin-4 (but not interferon-γ) secretion. The recombinant bacillus Calmette-Guérin-immunized group also exhibited mild delayed-type hypersensitivity reaction and a high frequency of CD3+CD45RBlow-activated T cells, together with high titer of antiprincipal neutralizing determinant immunoglobulin G antibodies. Thus, this epitope delivery system induced strong epitope-specific T-h-2 polarization.

Original language	English (US)
Pages (from-to)	45-55
Number of pages	11
Journal	FEMS Immunology and Medical Microbiology
Volume	47
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-695X.2006.00061.x
State	Published - Jun 2006

Keywords

Chaperonin-10 (Cpn10) promoter
Cryptic epitopes
Epitope-trap vector
HIV-1 principal neutralizing determinant (PND)
Recombinant bacillus Calmette-Guérin (r-BCG)

ASJC Scopus subject areas

Immunology and Allergy
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/j.1574-695X.2006.00061.x

Cite this

Aravindhan, V., Narayanan, S., Gautham, N., Prasad, V., Kannan, P., Jacobs, W. R., & Narayanan, P. R. (2006). T-h-2 immunity and CD3⁺ CD45RB^low-activated T cells in mice immunized with recombinant bacillus Calmette-Guérin expressing HIV-1 principal neutralizing determinant epitope. FEMS Immunology and Medical Microbiology, 47(1), 45-55. https://doi.org/10.1111/j.1574-695X.2006.00061.x

T-h-2 immunity and CD3⁺ CD45RB^low-activated T cells in mice immunized with recombinant bacillus Calmette-Guérin expressing HIV-1 principal neutralizing determinant epitope. / Aravindhan, Vivekanandhan; Narayanan, Sujatha; Gautham, Namasivayam et al.
In: FEMS Immunology and Medical Microbiology, Vol. 47, No. 1, 06.2006, p. 45-55.

Research output: Contribution to journal › Article › peer-review

Aravindhan, V, Narayanan, S, Gautham, N, Prasad, V, Kannan, P, Jacobs, WR & Narayanan, PR 2006, 'T-h-2 immunity and CD3⁺ CD45RB^low-activated T cells in mice immunized with recombinant bacillus Calmette-Guérin expressing HIV-1 principal neutralizing determinant epitope', FEMS Immunology and Medical Microbiology, vol. 47, no. 1, pp. 45-55. https://doi.org/10.1111/j.1574-695X.2006.00061.x

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