TY - JOUR
T1 - Studies with the schistosomacide hycanthone
T2 - Inhibition of macromolecular synthesis and its reversal
AU - Wittner, M.
AU - Tanowitz, Herbert
AU - Rosenbaum, Robert M.
PY - 1971/2
Y1 - 1971/2
N2 - The new schistosomacide, hycanthone, was studied with regard to its effects upon macromolecular synthesis in mammalian (HeLa) cells. At concentrations as low as 5 × 10-8 M, hycanthone reduces plating efficiency of HeLa cells. At 10-6M, effects on population growth are evident by the second generation. At 5 × 10-6 M, RNA synthesis is markedly depressed while DNA and protein synthesis are relatively unaffected. New ribosomal RNA synthesis is rapidly inhibited by hycanthone at 5 × 10-6 M, and its processing is markedly delayed. During hycanthone inhibition 28S RNA does not appear in the cytoplasm. Hycanthone inhibition was shown to be readily reversible by washing the cells in fresh medium. The case with which inhibition can be reversed in vitro suggests that it may be necessary to maintain blood levels of this compound if permanent eradication of schistosomal infection is to be attained. Further work must be done to establish this point.
AB - The new schistosomacide, hycanthone, was studied with regard to its effects upon macromolecular synthesis in mammalian (HeLa) cells. At concentrations as low as 5 × 10-8 M, hycanthone reduces plating efficiency of HeLa cells. At 10-6M, effects on population growth are evident by the second generation. At 5 × 10-6 M, RNA synthesis is markedly depressed while DNA and protein synthesis are relatively unaffected. New ribosomal RNA synthesis is rapidly inhibited by hycanthone at 5 × 10-6 M, and its processing is markedly delayed. During hycanthone inhibition 28S RNA does not appear in the cytoplasm. Hycanthone inhibition was shown to be readily reversible by washing the cells in fresh medium. The case with which inhibition can be reversed in vitro suggests that it may be necessary to maintain blood levels of this compound if permanent eradication of schistosomal infection is to be attained. Further work must be done to establish this point.
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U2 - 10.1016/0014-4800(71)90058-X
DO - 10.1016/0014-4800(71)90058-X
M3 - Article
C2 - 5549216
AN - SCOPUS:0015010292
SN - 0014-4800
VL - 14
SP - 124
EP - 133
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
IS - 1
ER -