TY - JOUR
T1 - Studies on the intranuclear distribution and properties of mouse satellite DNA
AU - Schildkraut, Carl L.
AU - Maio, Joseph J.
N1 - Funding Information:
Mr. ERIC BROWN. Miss GISELA JENTZSCI-I and Mr. STANLEY KLEIN prepared the electron micrograph. These studies were supported by the National Institutes of Health (AI-4153), the National Science Foundation and the American Cancer Society. One of us (C.L.S.) is a Kennedy Scholar.
PY - 1968/6/18
Y1 - 1968/6/18
N2 - Nucleoli isolated from mouse strain L-cells, L-5178Y lymphoblast tumor cells, and mouse liver and kidney cells showed a 3-4-fold enrichment in satellite DNA when compared with DNA from whole nuclei. This amount of satellite DNA associated with the nucleoli accounted for approximately one-third of the total satellite DNA of the cell. Treating the nucleoli with 2 M NaCl removed more main band DNA than satellite, and suggested that satellite DNA was more intimately bound to the nucleoli than main band DNA. However, attempts to demonstrate whether the satellite enrichment could have been due to preferential adsorption to the nucleoli during their isolation were inconclusive. Incubation of native or renatured satellite DNA with Escherichia coli exonuclease I did not produce any change in its buoyant density in CsCl. The base composition of satellite DNA was determined by using three different combinations of enzymes. Incubation with E. coli exonuclease III produced an increase in CsCl buoyant density and converted 45% of the satellite DNA to mononucleotides having the same average base composition as the total DNA. The satellite DNA did not behave differently from the main band DNA towards the deoxyribonucleases used in this study.
AB - Nucleoli isolated from mouse strain L-cells, L-5178Y lymphoblast tumor cells, and mouse liver and kidney cells showed a 3-4-fold enrichment in satellite DNA when compared with DNA from whole nuclei. This amount of satellite DNA associated with the nucleoli accounted for approximately one-third of the total satellite DNA of the cell. Treating the nucleoli with 2 M NaCl removed more main band DNA than satellite, and suggested that satellite DNA was more intimately bound to the nucleoli than main band DNA. However, attempts to demonstrate whether the satellite enrichment could have been due to preferential adsorption to the nucleoli during their isolation were inconclusive. Incubation of native or renatured satellite DNA with Escherichia coli exonuclease I did not produce any change in its buoyant density in CsCl. The base composition of satellite DNA was determined by using three different combinations of enzymes. Incubation with E. coli exonuclease III produced an increase in CsCl buoyant density and converted 45% of the satellite DNA to mononucleotides having the same average base composition as the total DNA. The satellite DNA did not behave differently from the main band DNA towards the deoxyribonucleases used in this study.
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U2 - 10.1016/0005-2787(68)90296-7
DO - 10.1016/0005-2787(68)90296-7
M3 - Article
C2 - 5690801
AN - SCOPUS:0014422181
SN - 0005-2787
VL - 161
SP - 76
EP - 93
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 1
ER -