Structural Changes in TAF4b-TFIID Correlate with Promoter Selectivity

Wei Li Liu; Robert A. Coleman; Patricia Grob; David S. King; Laurence Florens; Michael P. Washburn; Kenneth G. Geles; Joyce L. Yang; Vincent Ramey; Eva Nogales; Robert Tjian

doi:10.1016/j.molcel.2007.11.003

Structural Changes in TAF4b-TFIID Correlate with Promoter Selectivity

Wei Li Liu, Robert A. Coleman, Patricia Grob, David S. King, Laurence Florens, Michael P. Washburn, Kenneth G. Geles, Joyce L. Yang, Vincent Ramey, Eva Nogales, Robert Tjian

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Proper ovarian development requires the cell type-specific transcription factor TAF4b, a subunit of the core promoter recognition complex TFIID. We present the 35 Å structure of a cell type-specific core promoter recognition complex containing TAF4b and TAF4 (4b/4-IID), which is responsible for directing transcriptional synergy between c-Jun and Sp1 at a TAF4b target promoter. As a first step toward correlating potential structure/function relationships of the prototypic TFIID versus 4b/4-IID, we have compared their 3D structures by electron microscopy and single-particle reconstruction. These studies reveal that TAF4b incorporation into TFIID induces an open conformation at the lobe involved in TFIIA and putative activator interactions. Importantly, this open conformation correlates with differential activator-dependent transcription and promoter recognition by 4b/4-IID. By combining functional and structural analysis, we find that distinct localized structural changes in a megadalton macromolecular assembly can significantly alter its activity and lead to a TAF4b-induced reprogramming of promoter specificity.

Original language	English (US)
Pages (from-to)	81-91
Number of pages	11
Journal	Molecular Cell
Volume	29
Issue number	1
DOIs	https://doi.org/10.1016/j.molcel.2007.11.003
State	Published - Jan 18 2008
Externally published	Yes

Keywords

DEVBIO
DNA

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/j.molcel.2007.11.003

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@article{73821c7907d84ea791d2a2487f68fbc5,

title = "Structural Changes in TAF4b-TFIID Correlate with Promoter Selectivity",

abstract = "Proper ovarian development requires the cell type-specific transcription factor TAF4b, a subunit of the core promoter recognition complex TFIID. We present the 35 {\AA} structure of a cell type-specific core promoter recognition complex containing TAF4b and TAF4 (4b/4-IID), which is responsible for directing transcriptional synergy between c-Jun and Sp1 at a TAF4b target promoter. As a first step toward correlating potential structure/function relationships of the prototypic TFIID versus 4b/4-IID, we have compared their 3D structures by electron microscopy and single-particle reconstruction. These studies reveal that TAF4b incorporation into TFIID induces an open conformation at the lobe involved in TFIIA and putative activator interactions. Importantly, this open conformation correlates with differential activator-dependent transcription and promoter recognition by 4b/4-IID. By combining functional and structural analysis, we find that distinct localized structural changes in a megadalton macromolecular assembly can significantly alter its activity and lead to a TAF4b-induced reprogramming of promoter specificity.",

keywords = "DEVBIO, DNA",

author = "Liu, {Wei Li} and Coleman, {Robert A.} and Patricia Grob and King, {David S.} and Laurence Florens and Washburn, {Michael P.} and Geles, {Kenneth G.} and Yang, {Joyce L.} and Vincent Ramey and Eva Nogales and Robert Tjian",

note = "Funding Information: We thank S. Zheng for TAF4b antibody generation work, the Tjian lab tissue culture facility technicians, C. Inouye for purifying the basal factors, M. Haggart for assistance, and S. Zhou (HHMI, UC-Berkeley) for mass spectrometry. We also thank S. Lipscomb, A. Leschziner, H. Wang, and B. Siridechadilok for helpful advice and technical support. We are grateful to D. Reinberg, J.A. Goodrich, Z. Zhang, and U. Schulze-Gahmen for critical comments on the manuscript. This work was supported by NIH National Cancer Institute CA 25417 and PO1 CA112181 (R.T.), by NIH General Medical Sciences (E.N.), and by the U.S. Department of Energy in contract with the University of California (E.N.). R.T. and E.N. are Howard Hughes Medical Institute Investigators. R.T. is Director of the Li Ka-Shing Center for Biomedical and Health Sciences. ",

year = "2008",

month = jan,

day = "18",

doi = "10.1016/j.molcel.2007.11.003",

language = "English (US)",

volume = "29",

pages = "81--91",

journal = "Molecular Cell",

issn = "1097-2765",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Structural Changes in TAF4b-TFIID Correlate with Promoter Selectivity

AU - Liu, Wei Li

AU - Coleman, Robert A.

AU - Grob, Patricia

AU - King, David S.

AU - Florens, Laurence

AU - Washburn, Michael P.

AU - Geles, Kenneth G.

AU - Yang, Joyce L.

AU - Ramey, Vincent

AU - Nogales, Eva

AU - Tjian, Robert

N1 - Funding Information: We thank S. Zheng for TAF4b antibody generation work, the Tjian lab tissue culture facility technicians, C. Inouye for purifying the basal factors, M. Haggart for assistance, and S. Zhou (HHMI, UC-Berkeley) for mass spectrometry. We also thank S. Lipscomb, A. Leschziner, H. Wang, and B. Siridechadilok for helpful advice and technical support. We are grateful to D. Reinberg, J.A. Goodrich, Z. Zhang, and U. Schulze-Gahmen for critical comments on the manuscript. This work was supported by NIH National Cancer Institute CA 25417 and PO1 CA112181 (R.T.), by NIH General Medical Sciences (E.N.), and by the U.S. Department of Energy in contract with the University of California (E.N.). R.T. and E.N. are Howard Hughes Medical Institute Investigators. R.T. is Director of the Li Ka-Shing Center for Biomedical and Health Sciences.

PY - 2008/1/18

Y1 - 2008/1/18

N2 - Proper ovarian development requires the cell type-specific transcription factor TAF4b, a subunit of the core promoter recognition complex TFIID. We present the 35 Å structure of a cell type-specific core promoter recognition complex containing TAF4b and TAF4 (4b/4-IID), which is responsible for directing transcriptional synergy between c-Jun and Sp1 at a TAF4b target promoter. As a first step toward correlating potential structure/function relationships of the prototypic TFIID versus 4b/4-IID, we have compared their 3D structures by electron microscopy and single-particle reconstruction. These studies reveal that TAF4b incorporation into TFIID induces an open conformation at the lobe involved in TFIIA and putative activator interactions. Importantly, this open conformation correlates with differential activator-dependent transcription and promoter recognition by 4b/4-IID. By combining functional and structural analysis, we find that distinct localized structural changes in a megadalton macromolecular assembly can significantly alter its activity and lead to a TAF4b-induced reprogramming of promoter specificity.

AB - Proper ovarian development requires the cell type-specific transcription factor TAF4b, a subunit of the core promoter recognition complex TFIID. We present the 35 Å structure of a cell type-specific core promoter recognition complex containing TAF4b and TAF4 (4b/4-IID), which is responsible for directing transcriptional synergy between c-Jun and Sp1 at a TAF4b target promoter. As a first step toward correlating potential structure/function relationships of the prototypic TFIID versus 4b/4-IID, we have compared their 3D structures by electron microscopy and single-particle reconstruction. These studies reveal that TAF4b incorporation into TFIID induces an open conformation at the lobe involved in TFIIA and putative activator interactions. Importantly, this open conformation correlates with differential activator-dependent transcription and promoter recognition by 4b/4-IID. By combining functional and structural analysis, we find that distinct localized structural changes in a megadalton macromolecular assembly can significantly alter its activity and lead to a TAF4b-induced reprogramming of promoter specificity.

KW - DEVBIO

KW - DNA

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U2 - 10.1016/j.molcel.2007.11.003

DO - 10.1016/j.molcel.2007.11.003

M3 - Article

C2 - 18206971

AN - SCOPUS:38349074012

SN - 1097-2765

VL - 29

SP - 81

EP - 91

JO - Molecular Cell

JF - Molecular Cell

IS - 1

ER -

Structural Changes in TAF4b-TFIID Correlate with Promoter Selectivity

Abstract

Keywords

ASJC Scopus subject areas

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