TY - JOUR
T1 - Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells
AU - Darzacq, Xavier
AU - Kittur, Nupur
AU - Roy, Sujayita
AU - Shav-Tal, Yaron
AU - Singer, Robert H.
AU - Meier, U. Thomas
PY - 2006/4/24
Y1 - 2006/4/24
N2 - Mammalian H/ACA RNPs are essential for ribosome biogenesis, premessenger RNA splicing, and telomere maintenance. These RNPs consist of four core proteins and one RNA, but it is not known how they assemble. By interrogating the site of H/ACA RNA transcription, we dissected their biogenesis in single cells and delineated the role of the non-core protein NAF1 in the process. NAF1 and all of the core proteins except GAR1 are recruited to the site of transcription. NAF1 binds one of the core proteins, NAP57, and shuttles between nucleus and cytoplasm. Both proteins are essential for stable H/ACA RNA accumulation. NAF1 and GAR1 bind NAP57 competitively, suggesting a sequential interaction. Our analyses indicate that NAF1 binds NAP57 and escorts it to the nascent H/ACA RNA and that GAR1 then replaces NAF1 to yield mature H/ACA RNPs in Cajal bodies and nucleoli.
AB - Mammalian H/ACA RNPs are essential for ribosome biogenesis, premessenger RNA splicing, and telomere maintenance. These RNPs consist of four core proteins and one RNA, but it is not known how they assemble. By interrogating the site of H/ACA RNA transcription, we dissected their biogenesis in single cells and delineated the role of the non-core protein NAF1 in the process. NAF1 and all of the core proteins except GAR1 are recruited to the site of transcription. NAF1 binds one of the core proteins, NAP57, and shuttles between nucleus and cytoplasm. Both proteins are essential for stable H/ACA RNA accumulation. NAF1 and GAR1 bind NAP57 competitively, suggesting a sequential interaction. Our analyses indicate that NAF1 binds NAP57 and escorts it to the nascent H/ACA RNA and that GAR1 then replaces NAF1 to yield mature H/ACA RNPs in Cajal bodies and nucleoli.
UR - http://www.scopus.com/inward/record.url?scp=33646076438&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646076438&partnerID=8YFLogxK
U2 - 10.1083/jcb.200601105
DO - 10.1083/jcb.200601105
M3 - Article
C2 - 16618814
AN - SCOPUS:33646076438
SN - 0021-9525
VL - 173
SP - 207
EP - 218
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 2
ER -