Stable lentiviral vector-mediated gene silencing in human monocytic cell lines

Jimmy S. Lee, Neil E. Reiner

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations


A major obstacle in studying monocyte cell biology has been the resistance of these cells to genetic manipulation, particularly when using nonviral methods. In the approach outlined in this chapter, we describe a strategy for stable gene silencing of monocytic cells. Using a vesicular stomatitis virus (VSV)-pseudotyped lentiviral vector expressing short hairpin RNA (shRNA), stable silencing of the p110α isoform of PI3-kinase in the human monocytic cell lines THP-1 and U-937 was achieved.

Original languageEnglish (US)
Title of host publicationMacrophages and Dendritic Cells
Subtitle of host publicationMethods and Protocols
EditorsNeil Reiner
Number of pages14
StatePublished - Dec 1 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Lentiviral vector
  • Monocyte
  • PI3-kinase
  • RNAi
  • Transduction
  • shRNA
  • siRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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