TY - JOUR
T1 - Spatial regulation of expanded transcription in the Drosophila wing imaginal disc
AU - Wang, Lan Hsin
AU - Baker, Nicholas E.
N1 - Funding Information:
LHW received funding from the Ministry of Science and Technology of Taiwan (MOST 105-2311-B-016-001-MY2; MOST106-3111-Y-016-005) and the Ministry of National Defense-Medical Affairs Bureau, Taiwan (MAB-107-092). Research in NEB’s laboratory was supported by a grant from the NIH (GM047892). Leica Confocal Imaging was performed in the Analytical Imaging Facility at AECOM supported by the NCI (P30CA013330). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript We thank Hugo Bellen, Claude Desplan, and Laura Johnston for fly strains. Leica Confocal Imaging was performed in the Analytical Imaging Facility at AECOM. Zeiss confocal microscopy was performed at Neuroscience Program of Academia Sinica (NPAS), Taiwan. Stocks obtained from the Bloomington Drosophila Stock Center (BDSC) were used in this study.
Publisher Copyright:
© 2018 Wang, Baker. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2018/7
Y1 - 2018/7
N2 - Growth and patterning are coordinated during development to define organ size and shape. The growth, proliferation and differentiation of Drosophila wings are regulated by several conserved signaling pathways. Here, we show that the Salvador-Warts-Hippo (SWH) and Notch pathways converge on an enhancer in the expanded (ex) gene, which also responds to levels of the bHLH transcription factor Daughterless (Da). Separate cis-regulatory elements respond to Salvador-Warts-Hippo (SWH) and Notch pathways, to bHLH proteins, and to unidentified factors that repress ex transcription in the wing pouch and in the proneural region at the anterior wing margin. Senseless, a zinc-finger transcription factor acting in proneural regions, had a negative impact on ex transcription in the proneural region, but the transcriptional repressor Hairy had no effect. Our study suggests that a complex pattern of ex transcription results from integration of a uniform SWH signal with multiple other inputs, rather than from a pattern of SWH signaling.
AB - Growth and patterning are coordinated during development to define organ size and shape. The growth, proliferation and differentiation of Drosophila wings are regulated by several conserved signaling pathways. Here, we show that the Salvador-Warts-Hippo (SWH) and Notch pathways converge on an enhancer in the expanded (ex) gene, which also responds to levels of the bHLH transcription factor Daughterless (Da). Separate cis-regulatory elements respond to Salvador-Warts-Hippo (SWH) and Notch pathways, to bHLH proteins, and to unidentified factors that repress ex transcription in the wing pouch and in the proneural region at the anterior wing margin. Senseless, a zinc-finger transcription factor acting in proneural regions, had a negative impact on ex transcription in the proneural region, but the transcriptional repressor Hairy had no effect. Our study suggests that a complex pattern of ex transcription results from integration of a uniform SWH signal with multiple other inputs, rather than from a pattern of SWH signaling.
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U2 - 10.1371/journal.pone.0201317
DO - 10.1371/journal.pone.0201317
M3 - Article
C2 - 30063727
AN - SCOPUS:85050804606
SN - 1932-6203
VL - 13
JO - PloS one
JF - PloS one
IS - 7
M1 - e0201317
ER -