Solid state yellow and orange lasers for flow cytometry

Veena Kapoor, Vladimir Karpov, Claudette Linton, Fedor V. Subach, Vladislav V. Verkhusha, William G. Telford

Research output: Contribution to journalArticlepeer-review

68 Scopus citations


Diode and DPSS lasers emitting a variety of wavelengths are now commonly incorporated into flow cytometers, greatly increasing our capacity to excite a wide variety of fluorochromes. Until recently, however, virtually no practical technology existed for generating yellow or orange laser light for flow cytometry that was compatible with smaller instrumentation. In this study, we evaluate several new solid state laser systems that emit from the 570 to 600 nm as excitation sources for flow cytometry. DPSS 580, 589, and 592 nm sources were integrated into a cuvette-based flow cytometer (BD LSR II) and a stream-in-air cell sorter (FACSVantage DiVa), and used to excite a variety of yellow, orange, and red excited fluorochromes, including Texas Red, APC, and its tandem conjugates, and the genetically encoded red fluorescent protein HcRed and the more recently developed Katushka. All laser sources were successfully incorporated into the indicated flow cytometry platforms. The yellow and orange sources (particularly 592 nm) were ideal for exciting Texas Red, and provided excitation of APC and its tandems that was comparable to a traditional red laser source, albeit at higher power levels than red sources. Yellow and orange laser light was optimal for exciting HcRed and Katushka. Practical yellow and orange laser sources are now available for flow cytometry. This technology fills an important gap in the laser wavelengths available for flow, now almost any fluorochrome requiring visible light excitation can be accommodated.

Original languageEnglish (US)
Pages (from-to)570-577
Number of pages8
JournalCytometry Part A
Issue number6
StatePublished - Jun 2008


  • Allophycocyanin
  • Diode pumped solid state
  • HcRed
  • Katushka
  • Orange laser
  • Red fluorescent protein
  • Texas Red
  • Yellow laser

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology


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