Sex-biased effects on hippocampal circuit development by perinatal SERT expression in CA3 pyramidal neurons

Roberto De Gregorio, Galadu Subah, Jennifer C. Chan, Luisa Speranza, Xiaolei Zhang, Aarthi Ramakrishnan, Li Shen, Ian Maze, Patric K. Stanton, Ji Y. Sze

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Neurodevelopmental disorders ranging from autism to intellectual disability display sex-biased prevalence and phenotypical presentations. Despite increasing knowledge about temporospatial cortical map development and genetic variants linked to neurodevelopmental disorders, when and how sex-biased neural circuit derailment may arise in diseased brain remain unknown. Here, we identify in mice that serotonin uptake transporter (SERT) in non-serotonergic neurons – hippocampal and prefrontal pyramidal neurons – confers sex-biased effects specifically during neural circuit development. A set of gradient-patterned CA3 pyramidal neurons transiently express SERT to clear extracellular serotonin, coinciding with hippocampal synaptic circuit establishment. Ablating pyramidal neuron SERT (SERTPyramidΔ) alters dendritic spine developmental trajectory in the hippocampus, and precipitates sex-biased impairments in long-term activity-dependent hippocampal synaptic plasticity and cognitive behaviors. Transcriptomic analyses identify sex-biased alterations in gene sets associated with autism, dendritic spine structure, synaptic function and male-specific enrichment of dysregulated genes in glial cells in early postnatal SERTPyramidΔ hippocampus. Our data suggest that SERT function in these pyramidal neurons underscores a temporal- and brain region-specific regulation of normal sex-dimorphic circuit development and a source for sex-biased vulnerability to cognitive and behavioral impairments.

Original languageEnglish (US)
Article numberdev200549
JournalDevelopment (Cambridge)
Issue number21
StatePublished - Nov 2022


  • Circuit assembly
  • Hippocampus
  • SERT
  • Sex-biased circuit plasticity
  • Sex-biased genetic programming
  • Trophic serotonin

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology


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