TY - JOUR
T1 - Separation and quantification by gas chromatography-mass spectrometry of arabinitol enantiomers to aid the differential diagnosis of disseminated candidiasis
AU - Roboz, John
AU - Nieves, Edward
AU - Holland, J. F.
N1 - Funding Information:
This work was supportedb y a grant from the Departmento f Health and Human Services( CA40050-01)a nd by the T. J. Martell Memorial Foundation for Leukemia,C ancer and AIDS Research.T hanks are due to Dr. E. Bottone and his staff for their assistancew ith the microbiologicala spectso f this work.
PY - 1990/2/2
Y1 - 1990/2/2
N2 - To differentiate increased arabinitol due to fungal (only d-arabinitol) and nonfungal origin, O-trifluoroacetyl derivatives of the enantiomers were separated using α-perpentylated cyclodextrin columns and measured by selected ion monitoring. Mean±S.D. d/l in normal serum: 1.40±0.42. l/l ratios > 2.24, defined as normal mean+2S.D., were considered outside normal range. D/L was > 2.2 in 10 of 12 confirmed candidiasis cases with one false negative and one borderline. Renal dysfunction without candidiasis yielded normal d/l despite high arabinitol concentrations. d/l in normal urine was nearly identical to that in serum despite 60 times larger concentration. d/l ratios, determined by peak heights or areas, could be used without the need to determine concentrations.
AB - To differentiate increased arabinitol due to fungal (only d-arabinitol) and nonfungal origin, O-trifluoroacetyl derivatives of the enantiomers were separated using α-perpentylated cyclodextrin columns and measured by selected ion monitoring. Mean±S.D. d/l in normal serum: 1.40±0.42. l/l ratios > 2.24, defined as normal mean+2S.D., were considered outside normal range. D/L was > 2.2 in 10 of 12 confirmed candidiasis cases with one false negative and one borderline. Renal dysfunction without candidiasis yielded normal d/l despite high arabinitol concentrations. d/l in normal urine was nearly identical to that in serum despite 60 times larger concentration. d/l ratios, determined by peak heights or areas, could be used without the need to determine concentrations.
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U2 - 10.1016/S0021-9673(00)96082-5
DO - 10.1016/S0021-9673(00)96082-5
M3 - Article
C2 - 2329144
AN - SCOPUS:0025096951
SN - 0021-9673
VL - 500
SP - 413
EP - 426
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -