TY - JOUR
T1 - Replication and persistence of HPV DNA in cultured cells derived from laryngeal papillomas
AU - DiLorenzo, Teresa P.
AU - Taichman, Lorne B.
AU - Steinberg, Bettie M.
PY - 1992/1
Y1 - 1992/1
N2 - We have investigated the replication and persistence of human papillomavirus (HPV) type 6 and 11 DNA in cultured cells derived from laryngeal papillomas, with paradoxical findings. Measured by bromodeoxyuridine incorporation into heavy/light DNA separated on a cesium chloride gradient, viral DNA replicates in both primary and secondary cells. The ratio of the fraction of replicated viral to replicated cellular DNA was equal to or greater than 1 in all but one case and was closer to 2 in primary cells. Despite this efficient replication, HPV DNA is rapidly lost from the cells with passage. We propose that infected cells, or those with a high HPV copy number, show a selective decrease in plating efficiency compared to uninfected cells or those with a low copy number, which explains the loss of HPV DNA with repeated passage.
AB - We have investigated the replication and persistence of human papillomavirus (HPV) type 6 and 11 DNA in cultured cells derived from laryngeal papillomas, with paradoxical findings. Measured by bromodeoxyuridine incorporation into heavy/light DNA separated on a cesium chloride gradient, viral DNA replicates in both primary and secondary cells. The ratio of the fraction of replicated viral to replicated cellular DNA was equal to or greater than 1 in all but one case and was closer to 2 in primary cells. Despite this efficient replication, HPV DNA is rapidly lost from the cells with passage. We propose that infected cells, or those with a high HPV copy number, show a selective decrease in plating efficiency compared to uninfected cells or those with a low copy number, which explains the loss of HPV DNA with repeated passage.
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U2 - 10.1016/0042-6822(92)90068-Z
DO - 10.1016/0042-6822(92)90068-Z
M3 - Article
C2 - 1309272
AN - SCOPUS:0026516707
SN - 0042-6822
VL - 186
SP - 148
EP - 153
JO - Virology
JF - Virology
IS - 1
ER -