TY - JOUR
T1 - Regulation of interleukin 2-driven T-lymphocyte proliferation by prolactin
AU - Clevenger, Charles V.
AU - Russell, Diane H.
AU - Appasamy, Pierette M.
AU - Prystowsky, Michael B.
PY - 1990/8
Y1 - 1990/8
N2 - The requirement for prolactin in interleukin 2-driven T-cell proliferation was evaluated. Addition of an anti-prolactin antiserum resulted in the specific inhibition of T-cell proliferation in a time- and dose-dependent manner. Synthesis of prolactin and its mRNA, however, did not occur during interleukin 2 stimulation. Instead, previously internalized prolactin, presumably from fetal bovine serum, appears to serve as the source of prolactin under serum-free conditions. A 7-fold increase in a prolactin receptor occurred as a function of cell cycle progression; accumulation of a 1.6-kilobase prolactin receptor mRNA increased ≈2-fold. Interleukin 2 stimulation induced the translocation of prolactin into the nucleus and prolactin receptor to the nuclear periphery. These data indicate that extracellular prolactin is requisite for T-cell proliferation and suggest that the effects of prolactin are exerted in the nucleus.
AB - The requirement for prolactin in interleukin 2-driven T-cell proliferation was evaluated. Addition of an anti-prolactin antiserum resulted in the specific inhibition of T-cell proliferation in a time- and dose-dependent manner. Synthesis of prolactin and its mRNA, however, did not occur during interleukin 2 stimulation. Instead, previously internalized prolactin, presumably from fetal bovine serum, appears to serve as the source of prolactin under serum-free conditions. A 7-fold increase in a prolactin receptor occurred as a function of cell cycle progression; accumulation of a 1.6-kilobase prolactin receptor mRNA increased ≈2-fold. Interleukin 2 stimulation induced the translocation of prolactin into the nucleus and prolactin receptor to the nuclear periphery. These data indicate that extracellular prolactin is requisite for T-cell proliferation and suggest that the effects of prolactin are exerted in the nucleus.
KW - Cell cycle
KW - Flow cytometry
KW - Nucleus
KW - Prolactin receptor
UR - http://www.scopus.com/inward/record.url?scp=0025108099&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025108099&partnerID=8YFLogxK
U2 - 10.1073/pnas.87.16.6460
DO - 10.1073/pnas.87.16.6460
M3 - Article
C2 - 2143585
AN - SCOPUS:0025108099
SN - 0027-8424
VL - 87
SP - 6460
EP - 6464
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 16
ER -