Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide

Yulia G. Ermakova, Dmitry S. Bilan, Mikhail E. Matlashov, Natalia M. Mishina, Ksenia N. Markvicheva, Oksana M. Subach, Fedor V. Subach, Ivan Bogeski, Markus Hoth, Grigori Enikolopov, Vsevolod V. Belousov

Research output: Contribution to journalArticlepeer-review

189 Scopus citations


Reactive oxygen species (ROS) are conserved regulators of numerous cellular functions, and overproduction of ROS is a hallmark of various pathological processes. Genetically encoded fluorescent probes are unique tools to study ROS production in living systems of different scale and complexity. However, the currently available recombinant redox sensors have green emission, which overlaps with the spectra of many other probes. Expanding the spectral range of recombinant in vivo ROS probes would enable multiparametric in vivo ROS detection. Here we present the first genetically encoded red fluorescent sensor for hydrogen peroxide detection, HyPerRed. The performance of this sensor is similar to its green analogues. We demonstrate the utility of the sensor by tracing low concentrations of H2O2 produced in the cytoplasm of cultured cells upon growth factor stimulation. Moreover, using HyPerRed we detect local and transient H2O2 production in the mitochondrial matrix upon inhibition of the endoplasmic reticulum Ca2+ uptake.

Original languageEnglish (US)
Article number5222
JournalNature communications
StatePublished - 2014
Externally publishedYes

ASJC Scopus subject areas

  • General Chemistry
  • General Biochemistry, Genetics and Molecular Biology
  • General Physics and Astronomy


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