Recombinant fluorescent models for studying of diphtheria toxin

A. Iu Labyntsev, N. V. Korotkevich, K. Iu Manoĭlov, A. A. Kaberniuk, D. V. Kolibo, S. V. Komisarenko

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Diphtheria toxin is the main pathogenicity factor of causative agent of diphtheria Corynebacterium diphtheriae. Due to the small molecule size, it is of considerable interestfor the development of synthetic protein molecules with transporting function, e.g. immunotoxins. Expression and characterization of nontoxic recombinant fluorescent derivates of diphtheria toxin and its nontoxic mutant CRM 197 were described in this article. Obtained proteins may be applied in studies of receptor-binding and transporting functions of the toxin in cells, for determination of toxin receptor proHB-EGF expression level, immunization and antibody generation against the toxin and in development of diagnostic test-systems, detection of diphtheria toxin and antitoxic antibodies.

Original languageEnglish (US)
Pages (from-to)433-442
Number of pages10
JournalBioorganicheskaia khimiia
Issue number4
StatePublished - Jul 1 2014

ASJC Scopus subject areas

  • Medicine(all)


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