Rapid isolation of BMPR-IB+ adipose-derived stromal cells for use in a calvarial defect healing model

Clement D. Marshall, Elizabeth R. Zielins, Elizabeth A. Brett, Charles P. Blackshear, Michael S. Hu, Tripp Leavitt, Leandra A. Barnes, H. Peter Lorenz, Michael T. Longaker, Derrick C. Wan

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Invasive cancers, major injuries, and infection can cause bone defects that are too large to be reconstructed with preexisting bone from the patient's own body. The ability to grow bone de novo using a patient's own cells would allow bony defects to be filled with adequate tissue without the morbidity of harvesting native bone. There is interest in the use of adipose-derived stromal cells (ASCs) as a source for tissue engineering because these are obtained from an abundant source: the patient's own adipose tissue. However, ASCs are a heterogeneous population and some subpopulations may be more effective in this application than others. Isolation of the most osteogenic population of ASCs could improve the efficiency and effectiveness of a bone engineering process. In this protocol, ASCs are obtained from subcutaneous fat tissue from a human donor. The subpopulation of ASCs expressing the marker BMPR-IB is isolated using FACS. These cells are then applied to an in vivo calvarial defect healing assay and are found to have improved osteogenic regenerative potential compared with unsorted cells.

Original languageEnglish (US)
Article numbere55120
JournalJournal of Visualized Experiments
Issue number120
StatePublished - Feb 24 2017
Externally publishedYes


  • Adipose-derived stromal cell
  • Bone healing
  • Calvarial defect
  • Cell isolation
  • Developmental biology
  • FACS
  • Fat
  • Issue 120
  • Osteogenesis

ASJC Scopus subject areas

  • General Neuroscience
  • General Chemical Engineering
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology


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