TY - JOUR
T1 - Radioimmunoassay for the measurement of mass and avidity of anti-nerve growth factor antibodies
AU - Revoltella, Roberto
AU - Angeletti, Ruth Hogue
AU - Pediconi, Marcella
AU - Bertolini, Luisa
N1 - Funding Information:
* This research was supported by the Consiglio Nazionale delle Ricerche, Rome, Italy.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1974/1
Y1 - 1974/1
N2 - A radioimmunoassay is described for measuring on a weight basis the total amount of humoral antibodies to the purified protein nerve growth factor (NGF). The technique is based on the primary interaction of the antibodies with 125I-labelled NGF added in large molar excess. The resultant immune complexes are precipitated by ammonium sulfate at 37% saturation, while unbound 125I-labelled NGF is removed with the supernatant. The assay permits measurement of antibodies in a range of sensitivity of 0.1-1.0 ng antibody protein per ml. It is specific and reproducible and permits estimates of the antibody avidity.
AB - A radioimmunoassay is described for measuring on a weight basis the total amount of humoral antibodies to the purified protein nerve growth factor (NGF). The technique is based on the primary interaction of the antibodies with 125I-labelled NGF added in large molar excess. The resultant immune complexes are precipitated by ammonium sulfate at 37% saturation, while unbound 125I-labelled NGF is removed with the supernatant. The assay permits measurement of antibodies in a range of sensitivity of 0.1-1.0 ng antibody protein per ml. It is specific and reproducible and permits estimates of the antibody avidity.
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U2 - 10.1016/0022-1759(74)90035-0
DO - 10.1016/0022-1759(74)90035-0
M3 - Article
C2 - 4595765
AN - SCOPUS:0015955563
SN - 0022-1759
VL - 4
SP - 67
EP - 82
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -