Protocol for isolating single species of bacteria with swarming ability from human feces

Hao Li; Lilli Hong; Wendy Szymczak; Erika Orner; Arkadiy I. Garber; Vaughn S. Cooper; Weijie Chen; Arpan De; Jay X. Tang; Sridhar Mani

doi:10.1016/j.xpro.2024.102961

Protocol for isolating single species of bacteria with swarming ability from human feces

Hao Li, Lilli Hong, Wendy Szymczak, Erika Orner, Arkadiy I. Garber, Vaughn S. Cooper, Weijie Chen, Arpan De, Jay X. Tang, Sridhar Mani

Research output: Contribution to journal › Article › peer-review

Abstract

Understanding the specific movements of bacteria isolated from human feces can serve as a novel diagnostic and therapeutic tool for inflammatory bowel disease. Here, we present a protocol for a microbial swarming assay and to isolate the bacteria responsible for swarming activity. We describe steps for identifying bacteria using MALDI-TOF mass spectrometry and whole-genome sequencing. We then detail procedures for validating findings by observing the same swarming phenotype upon reperforming the swarming assay. For complete details on the use and execution of this protocol, please refer to De et al.¹

Original language	English (US)
Article number	102961
Journal	STAR Protocols
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2024.102961
State	Published - Jun 21 2024

Keywords

Health Sciences
Mass Spectrometry
Microbiology
Sequencing

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2024.102961

Cite this

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title = "Protocol for isolating single species of bacteria with swarming ability from human feces",

abstract = "Understanding the specific movements of bacteria isolated from human feces can serve as a novel diagnostic and therapeutic tool for inflammatory bowel disease. Here, we present a protocol for a microbial swarming assay and to isolate the bacteria responsible for swarming activity. We describe steps for identifying bacteria using MALDI-TOF mass spectrometry and whole-genome sequencing. We then detail procedures for validating findings by observing the same swarming phenotype upon reperforming the swarming assay. For complete details on the use and execution of this protocol, please refer to De et al.1",

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T1 - Protocol for isolating single species of bacteria with swarming ability from human feces

AU - Li, Hao

AU - Hong, Lilli

AU - Szymczak, Wendy

AU - Orner, Erika

AU - Garber, Arkadiy I.

AU - Cooper, Vaughn S.

AU - Chen, Weijie

AU - De, Arpan

AU - Tang, Jay X.

AU - Mani, Sridhar

PY - 2024/6/21

Y1 - 2024/6/21

N2 - Understanding the specific movements of bacteria isolated from human feces can serve as a novel diagnostic and therapeutic tool for inflammatory bowel disease. Here, we present a protocol for a microbial swarming assay and to isolate the bacteria responsible for swarming activity. We describe steps for identifying bacteria using MALDI-TOF mass spectrometry and whole-genome sequencing. We then detail procedures for validating findings by observing the same swarming phenotype upon reperforming the swarming assay. For complete details on the use and execution of this protocol, please refer to De et al.1

AB - Understanding the specific movements of bacteria isolated from human feces can serve as a novel diagnostic and therapeutic tool for inflammatory bowel disease. Here, we present a protocol for a microbial swarming assay and to isolate the bacteria responsible for swarming activity. We describe steps for identifying bacteria using MALDI-TOF mass spectrometry and whole-genome sequencing. We then detail procedures for validating findings by observing the same swarming phenotype upon reperforming the swarming assay. For complete details on the use and execution of this protocol, please refer to De et al.1

KW - Health Sciences

KW - Mass Spectrometry

KW - Microbiology

KW - Sequencing

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Protocol for isolating single species of bacteria with swarming ability from human feces

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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