TY - CHAP
T1 - Protein-ligand interactions of the D-galactose/D-glucose-binding protein as a potential sensing probe of glucose biosensors
AU - Stepanenko, Olga V.
AU - Stepanenko, Olesya V.
AU - Fonin, Alexander V.
AU - Verkhusha, Vladislav V.
AU - Kuznetsova, Irina M.
AU - Turoverov, Konstantin K.
PY - 2013
Y1 - 2013
N2 - In this work we have studied peculiarities of protein-ligand interaction under different conditions. We have shown that guanidine hydrochloride (GdnHCl) unfolding-refolding of GGBP in the presence of glucose (Glc) is reversible, but the equilibrium curves of complex refolding-unfolding have been attained only after 10 days incubation of GGBP/Glc in the presence of GdnHCl. This effect has not been revealed at heat-induced GGBP/Glc denaturation. Slow equilibration between the native protein in GGBP/Glc complex and the unfolded state of protein in the GdnHCl presence is connected with increased viscosity of solution at moderate and high GdnHCl concentrations which interferes with diffusion of glucose molecules. Thus, the limiting step of the unfolding-refolding process of the complex GGBP/Glc is the disruption/tuning of the configuration fit between the protein in the native state and the ligand.
AB - In this work we have studied peculiarities of protein-ligand interaction under different conditions. We have shown that guanidine hydrochloride (GdnHCl) unfolding-refolding of GGBP in the presence of glucose (Glc) is reversible, but the equilibrium curves of complex refolding-unfolding have been attained only after 10 days incubation of GGBP/Glc in the presence of GdnHCl. This effect has not been revealed at heat-induced GGBP/Glc denaturation. Slow equilibration between the native protein in GGBP/Glc complex and the unfolded state of protein in the GdnHCl presence is connected with increased viscosity of solution at moderate and high GdnHCl concentrations which interferes with diffusion of glucose molecules. Thus, the limiting step of the unfolding-refolding process of the complex GGBP/Glc is the disruption/tuning of the configuration fit between the protein in the native state and the ligand.
KW - D-galactose/D-glucose-binding protein
KW - biosensor system
KW - intrinsic fluorescence of proteins
KW - protein stability
KW - viscosity
UR - http://www.scopus.com/inward/record.url?scp=84888090839&partnerID=8YFLogxK
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U2 - 10.3233/978-1-61499-184-7-255
DO - 10.3233/978-1-61499-184-7-255
M3 - Chapter
AN - SCOPUS:84888090839
SN - 9781614991830
T3 - Advances in Biomedical Spectroscopy
SP - 255
EP - 260
BT - Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011
A2 - Marques, Maria
A2 - Batista de Carvalho, Luis
A2 - Haris, Parvez
ER -