Preparation of [35S]sulfobromophthalein of high specific activity

Hiroko Kurisu, Polly Nilprabhassorn, Allan W. Wolkoff

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


Study of the hepatocyte transport mechanism of organic anions such as bilirubin and sulfobromophthalein has been limited by the relatively low specific activities of these ligands. [3H]Bilirubin and [35S]sulfobromophthalein have been available with specific activities of only approximately 100 mCi/mmol. We now report a relatively simple procedure to prepare [35S]sulfobromophthalein at a specific activity of approximately 3000 mCi/mmol. This compound is radiochemically pure and serves as a tracer for authentic sulfobromophthalein as judged by chromatography, hepatocyte uptake, metabolism, and biliary excretion. Use of this material as a photoaffinity probe and as a transported ligand may permit dissection and understanding of its transport mechanism.

Original languageEnglish (US)
Pages (from-to)72-74
Number of pages3
JournalAnalytical Biochemistry
Issue number1
StatePublished - May 15 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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