TY - JOUR
T1 - Pores formed in lipid bilayer membranes by nystatin
T2 - Differences in its one-sided and two-sided action
AU - Marty, Alain
AU - Finkelstein, Alan
PY - 1975/4/1
Y1 - 1975/4/1
N2 - Nystatin and amphotericin B induce a cation-selective conductance when added to one side of a lipid bilayer membrane and an anion-selective conductance when added to both sides. The concentrations of antibiotic required for the one-sided action are comparable to those employed on plasma membranes and are considerably larger than those required for the two-sided action. We propose that the two-sided effect results from the formation of aqueous pores formed by the hydrogen bonding in the middle of the bilayer of two "half pores," whereas the one-sided effect results from the half pores alone. We discuss, in terms of the flexibility of bilayer structure and its thickness, how it is possible to have conducting half pores and "complete pores" in the same membrane. The role of sterol (cholesterol and ergosterol) in pore formation is also examined.
AB - Nystatin and amphotericin B induce a cation-selective conductance when added to one side of a lipid bilayer membrane and an anion-selective conductance when added to both sides. The concentrations of antibiotic required for the one-sided action are comparable to those employed on plasma membranes and are considerably larger than those required for the two-sided action. We propose that the two-sided effect results from the formation of aqueous pores formed by the hydrogen bonding in the middle of the bilayer of two "half pores," whereas the one-sided effect results from the half pores alone. We discuss, in terms of the flexibility of bilayer structure and its thickness, how it is possible to have conducting half pores and "complete pores" in the same membrane. The role of sterol (cholesterol and ergosterol) in pore formation is also examined.
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U2 - 10.1085/jgp.65.4.515
DO - 10.1085/jgp.65.4.515
M3 - Article
C2 - 1151324
AN - SCOPUS:0016670377
SN - 0022-1295
VL - 65
SP - 515
EP - 526
JO - Journal of General Physiology
JF - Journal of General Physiology
IS - 4
ER -