Abstract
We have used the polymerase chain reaction (PCR) to analyze the 50 base pair (bp) insertion/deletion polymorphism in the coagulation factor 9 gene. This procedure is particularly applicable for DNA marker studies in fragile X families. The polymorphism, which can also be detected in Dde I digestions, was detected by the amplification of fragments of 298 and 348 bp. The alleles were distinguished directly by agarose gel electrophoresis. PCR detection of this polymorphism is much simpler, more accurate, and quicker than conventional analysis.
Original language | English (US) |
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Pages (from-to) | 378-379 |
Number of pages | 2 |
Journal | American journal of medical genetics |
Volume | 38 |
Issue number | 2-3 |
DOIs | |
State | Published - 1991 |
Externally published | Yes |
Keywords
- DNA polymorphism
- linkage analysis
- restriction enzymes
ASJC Scopus subject areas
- Genetics(clinical)