Abstract
The herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) promoter contains elements involved in both constitutive and induced expression. We determined that phorbol 12-myristate 13-acetate (PMA) induces the HSV-1 TK promoter in HEK293 cells. However, PMA did not induce expression from the promoter in HeLa cells and did not result in a globally increased gene expression in HEK293 cells. Induction of HSV-1 TK promoter required activation of both of JNK and ERK pathways. However, activation of the two pathways alone was not sufficient for induction of HSV-1 TK promoter. By transiently transfecting into HeLa cells the adenoviral E1A gene, which exists as an integrant in HEK293 genome, we demonstrated that E1A proteins are necessary for induction of HSV-1 TK promoter by PMA. We propose mechanisms by which signaling pathways activated by the tumor-promoter PMA cooperate with the oncogene E1A to stimulate a eukaryotic promoter, namely the HSV-1 TK promoter.
Original language | English (US) |
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Pages (from-to) | 145-157 |
Number of pages | 13 |
Journal | Archives of Biochemistry and Biophysics |
Volume | 490 |
Issue number | 2 |
DOIs | |
State | Published - Oct 15 2009 |
Keywords
- E1A
- ERK
- JNK
- PMA
- Thymidine kinase
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology